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Cell surface expression of proteins using Pichia yeast: Development and application of high-throuput construction of expression system

Research Project

Project/Area Number 25450126
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied biochemistry
Research InstitutionKyoto University

Principal Investigator

Mizutani Kimihiko  京都大学, 農学研究科, 助教 (40314281)

Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsタンパク質工学 / Pichia pastoris / 発現系構築 / X線結晶構造解析
Outline of Final Research Achievements

Using High-throuput method constructing Pichia yeast protein expression system, cell surface expression of green fluorescence protein and the target protein using Pichia yeast has been achieved. Pichia yeast producing alpha-amylase on the cell surface had starch diesting activity.
Using Pichia yeast, cellulose digesting enzymes of molluscs were produced. For the enzyme of a snail, molecular structure and catalytic mechanism were analyzed.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (2 results)

All 2017 2013

All Presentation (2 results) (of which Invited: 1 results)

  • [Presentation] 貝類由来セルラーゼEG27II のX 線結晶構造解析および機能解析2017

    • Author(s)
      野村泰介、水谷公彦、岩瀬久夢、高橋延行、三上文三
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学
    • Related Report
      2016 Annual Research Report
  • [Presentation] 酵母発現系を用いたハイスループット構造生物学2013

    • Author(s)
      水谷公彦
    • Organizer
      日本農芸化学会関西支部例会
    • Place of Presentation
      大阪府立大学
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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