Mechanism of IgA-mediated cross protection induced by mucosal immunization

• Project/Area Number: 25450418
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Hokkaido University
• Principal Investigator: YOSHIDA Reiko 北海道大学, 人獣共通感染症リサーチセンター, 特任助教 (80435966)
• Co-Investigator(Renkei-kenkyūsha): TAKADA Ayato 北海道大学, 人獣共通感染症リサーチセンター, 教授 (10292062)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 粘膜免疫 / インフルエンザウイルス / IgA / 交差感染防御

Outline of Final Research Achievements

Mucosal immunization with influenza virus vaccine confers cross-protection against infection with influenza virus antigenic variants as well as multiple subtypes of influenza A viruses. In this study, we found that many cross-reactive but non-neutralizing anti-HA IgA antibodies are produced in the upper respiratory tract of immunized mice. These anti-HA IgA antibodies showed stronger antiviral activities against influenza A viruses than IgG antibodies. Particularly, the results from this study suggest that the inhibition of virus particles release from the infected cell surface may be one of the IgA-mediated protective mechanisms important for heterosubtypic immunity.

Research Products

• [Journal Article] Discovery of an antibody for pan-ebolavirus therapy (2016)
  • Author(s): Furuyama D, Marzi A, Nanbo A, Haddock E, Maruyama J, Miyamoto H, Igarashi M, Yoshida R, Dr. Noyori O, Feldmann H, Takada A
  • Journal Title: Sci Rep Volume: 6 Issue: 1 Pages: 20514-20514
  • DOI: 10.1038/srep20514
  • NAID: 120005906783
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of the glycoproteins of bat-derived influenza viruses. (2016)
  • Author(s): Maruyama J, Nao N, Miyamoto H, Maeda K, Ogawa H, Yoshida R, Igarashi M, Takada A
  • Journal Title: Virology Volume: 488 Pages: 43-50
  • DOI: 10.1016/j.virol.2015.11.002
  • NAID: 120005906991
  • Peer Reviewed / Open Access
• [Journal Article] A Single Amino Acid in the M1 Protein Responsible for the Different Pathogenic Potentials of H5N1 Highly Pathogenic Avian Influenza Virus Strains (2015)
  • Author(s): Nao N, Kajihara M, Manzoor R, Maruyama J, Yoshida R, Muramatsu M, Miyamoto H, Igarashi M, Eguchi N, Sato M, Kondoh T, Okamatsu M, Sakoda Y, Kida H, Takada A
  • Journal Title: PLoS One Volume: 10(9) Issue: 9 Pages: e0137989-e0137989
  • DOI: 10.1371/journal.pone.0137989
  • NAID: 120005672370
  • Peer Reviewed / Open Access
• [Journal Article] A polymorphism of the TIM-1 IgV domain: Implications for the susceptibility to filovirus infection. (2014)
  • Author(s): Makoto Kuroda, Daisuke Fujikura, Osamu Noyori, Masahiro Kajihara, Junki Maruyama, Hiroko Miyamoto, Reiko Yoshida, Ayato Takada.
  • Journal Title: Biochemical and biophysical research communications Volume: 455 Issue: 3-4 Pages: 223-228
  • DOI: 10.1016/j.bbrc.2014.10.144
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of Antiviral Activity between IgA and IgG Specific to Influenza Virus Hemagglutinin: Increased Potential of IgA for Heterosubtypic Immunity (2013)
  • Author(s): Muramatsu M, Yoshida R, Yokoyama A, Miyamoto H, Kajihara M, Maruyama J, Nao N, Manzoor R, Takada A
  • Journal Title: PLoS ONE Volume: 9 Issue: 1 Pages: e85582-e85582
  • DOI: 10.1371/journal.pone.0085582
  • NAID: 120005425353
  • Peer Reviewed
• [Journal Article] Heterosubtypic antiviral activity of hemagglutinin-specific antibodies induced by intranasal immunization with inactivated influenza viruses in mice. (2013)
  • Author(s): Muramatsu M. et al.
  • Journal Title: PLoS One Volume: 8 Issue: 8 Pages: e71534-e71534
  • DOI: 10.1371/journal.pone.0071534
  • Peer Reviewed
• [Presentation] Development of an immunochromatography rapid kit for Ebola virus antigen (2015)
  • Author(s): Reiko Yoshida，Shino Muramatsu，Hiroshi Akita，Yuji Saito，Miwa Kuwahara，Daisuke Kato2），Changula Katendi，Hiroko Miyamoto，Masahiro Kajihara，Ayato Takada
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2015-11-22
• [Presentation] An amino acid substitution in the glycoprotein of severe fever with (2015)
  • Author(s): Yoshimi Tsuda，Sanae Nishio，Ryo Ito，Reiko Yoshida，Ayato Takada，Jiro Arikawa，Kumiko Yoshimatu
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2015-11-22
• [Presentation] Characterization of the glycoproteins of bat-derived influenza viruses (2015)
  • Author(s): Junki Maruyama，Naganori Nao，Hiroko Miyamoto，Ken Maeda，Hirohito Ogawa，Reiko Yoshida，Manabu Igarashi，Ayato Takada
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2015-11-22
• [Presentation] Computational analysis of common epitope recognized by a broadly neutralizing (2015)
  • Author(s): Manabu Igarashi，Masakazu Sekijima，Nobuaki Yasuo，Takashi Abe，Reiko Yoshida，Teruaki Watabe，Erika Ishitsubo，Hiroaki Tokiwa，Takatsugu Hirokawa，Ayato Takada
  • Organizer: 第63回日本ウイルス学会学術集会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2015-11-22
• [Presentation] H5N1 インフルエンザウイルスに対する中和抗体を用いた受動免疫の感染防御効果 (2014)
  • Author(s): 吉田玲子、伊藤靖、七戸新太郎、小笠原一誠、日尾野隆大、岡松正敏、迫田義博、喜田宏、Mai Quynh Le、河岡義裕、五十嵐学、鈴木定彦、高田礼人
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-12
• [Presentation] A 型インフルエンザウイルスヘマグルチニン特異的IgA 抗体の亜型間交差抗ウイルス活性 (2014)
  • Author(s): 村松美笑子、吉田玲子、横山文香、宮本洋子、梶原将大、丸山隼輝、直享則、Rashid Manzoor、高田礼人
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-12
• [Presentation] Comparison of antiviral activity between IgA and IgG specific to influenza virushemagglutinin: increased potential of IgA for heterosubtypic immunity (2014)
  • Author(s): Mieko Muramatsu, Reiko Yoshida, Ayato Takada
  • Organizer: XVIth International Congress of Virology
  • Place of Presentation: Montréal’s Convention Centre (Canada)
  • Year and Date: 2014-07-28
• [Presentation] Heterosubtypic antiviral activity of influenza virus hemagglutinin-specific antibodies (2013)
  • Author(s): Mieko Muramatsu, Reiko Yoshida, Ayato Takada
  • Organizer: 15th International Negative Strand Virus Meeting
  • Place of Presentation: Palacio de Exposiciones y Congresos de Granada(Spain)
• [Remarks] 北海道大学 人獣共通感染症リサーチセンター 国際疫学部門
  • URL: http://www.czc.hokudai.ac.jp/epidemiol/
• [Remarks] 北海道大学 人獣共通感染症リサーチセンター 国際疫学部門
  • URL: http://www.czc.hokudai.ac.jp/epidemiol/
• [Remarks] 北海道大学人獣共通感染症リサーチセンター国際疫学部門
  • URL: http://www.czc.hokudai.ac.jp/research/epidemiology/