Structural heterogeneity of mammalian testis ribosomes: comprehensive analysis for new mechanisms of translational control

• Project/Area Number: 25450514
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Nagoya University
• Principal Investigator: NADANO Daita 名古屋大学, 生命農学研究科, 准教授 (00228074)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: リボソーム / 翻訳 / 発現制御

Outline of Final Research Achievements

Our proteomic analysis of rodent cytoplasmic ribosomes revealed their structural heterogeneity. This analysis identified a zinc finger protein, Lyar, in testicular ribosomes. Lyar was suggested to be included in the 60S large subunit, but not in polysomes, by ultracentrifugation of testicular ribosomes. Furthermore, translation was increased by Lyar in vitro, pointing out the involvement of this protein in translation. Overall, our data in this study (including unpublished ones) are expected to make a connection between ribosome heterogeneity and translational control.

Research Products

• [Journal Article] ふぞろいなリボソームの発見とその役割―新たな翻訳制御・癌化メカニズムとしての構造不均一性 (2016)
  • Author(s): 灘野大太・杉原圭彦
  • Journal Title: 化学と生物 Volume: 54 Pages: 77-79
  • Peer Reviewed
• [Journal Article] Lyar, a cell growth-regulating zinc finger protein, was identified to be associated with cytoplasmic ribosomes in male germ and cancer cells. (2014)
  • Author(s): Yonezawa, K., Sugihara, Y., Oshima, K., Matsuda, T., and Nadano, D.
  • Journal Title: Mol. Cell. Biochem. Volume: 395 Issue: 1-2 Pages: 221-229
  • DOI: 10.1007/s11010-014-2128-x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification and expression of an autosomal paralogue of ribosomal protein S4, X-linked, in mice: Potential involvement of testis-specific ribosomal proteins in translation and spermatogenesis. (2013)
  • Author(s): Sugihara, Y., Sadohara, E., Yonezawa, K., Kugo, M., Oshima, K., Matsuda, T., and Nadano, D.
  • Journal Title: Gene Volume: 521 Issue: 1 Pages: 91-99
  • DOI: 10.1016/j.gene.2013.02.040
  • Peer Reviewed
• [Presentation] 細胞増殖促進タンパク質Lyarのリボソーム不均一性因子としての同定 (2015)
  • Author(s): 米澤佳保里・杉原圭彦・大島健司・松田幹・灘野大太
  • Organizer: 第３回リボソームミーティング
  • Place of Presentation: 宮崎県宮崎市
  • Year and Date: 2015-03-17 – 2015-03-18
• [Remarks] 高等動物における翻訳（タンパク質合成）制御の解明
  • URL: http://www.agr.nagoya-u.ac.jp/~molreg00/translation.html
• [Remarks] 高等動物における翻訳（タンパク質合成）制御の解明
  • URL: http://www.agr.nagoya-u.ac.jp/~molreg00/translation.html