Studies on the mitochondrial proteins responsible for the traffic control across mitochondrial outer membrane, and its application for drug design

• Project/Area Number: 25460066
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: The University of Tokushima
• Principal Investigator: SHINOHARA Yasuo 徳島大学, 疾患プロテオゲノム研究センター, 教授 (60226157)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ミトコンドリア / 電位依存性アニオンチャネル / カルニチンパルミトイル転移酵素 / 外膜 / VDAC / CPT1 / 溶質輸送

Outline of Final Research Achievements

Mitochondrial outer membrane is highly permeable for various metabolites and ions. This high permeability is conferred by the voltage dependent anion channel expressed in the outer mitochondrial membrane. The carnitine palmitoyltransferase also present in the mitochondrial outer membrane is also responsible for the translocation of the fatty acids across mitochondrial membranes. In the present study, we focused on these two proteins, and tried to understand their structural and functional properties. These approaches are effective for better understanding of the properties of the mitochondrial outer membrane.

Research Products

• [Journal Article] Effects of employment of distinct strategies to capture antibody on antibody delivery into cultured cells. (2015)
  • Author(s): Kuwahara K, Harada K, Yamagoshi R, Yamamoto T, Shinohara Y.
  • Journal Title: Mol Cell Biochem Volume: 404 Issue: 1-2 Pages: 25-30
  • DOI: 10.1007/s11010-015-2362-x
  • NAID: 120006763927
  • Peer Reviewed
• [Journal Article] Bongkrekic Acid Analogue, Lacking One of the Carboxylic Groups of its Parent Compound, Shows Moderate but pH-insensitive Inhibitory Effects on the Mitochondrial ADP/ATP Carrier (2015)
  • Author(s): Atsushi Yamamoto, Keisuke Hasui, Hiroshi Matsuo, Katsuhiro Okuda, Masato Abe, Kenji Matsumoto, Kazuki Harada, Yuya Yoshimura, Takenori Yamamoto, Kazuto Ohkura, Mitsuru Shindo, Yasuo Shinohara
  • Journal Title: Chem. Biol. Drug Des. Volume: 86 Issue: 5 Pages: 1304-1322
  • DOI: 10.1111/cbdd.12594
  • NAID: 120006732016
  • Peer Reviewed / Open Access
• [Journal Article] Immunoblotting with Peptide Antibodies: Differential Immunoreactivities Caused by Certain Amino Acid Substitutions in a Short Peptide and Possible Effects of Differential Refolding of the Peptide on a Nitrocellulose or PVDF Membrane (2015)
  • Author(s): Takenori Yamamoto, Taisuke Matsuo, Atsushi Yamamoto, Ryohei Yamagoshi, Kazuto Ohkura, Masatoshi Kataoka, Yasuo Shinohara
  • Journal Title: Methods Mol. Biol. Volume: 1348 Pages: 303-310
  • DOI: 10.1007/978-1-4939-2999-3_26
  • ISBN: 9781493929986, 9781493929993
  • Peer Reviewed / Open Access
• [Journal Article] Utility of syntenic relationships of VDAC1 pseudogenes for not only an understanding of the phylogenetic divergence history of rodents, but also ascertaining possible pseudogene candidates as genuine pseudogenes (2014)
  • Author(s): Yusuke Ido, Tatsuki Yoshitomi, Kazuto Ohkura, Takenori Yamamoto, Yasuo Shinohara
  • Journal Title: Genomics Volume: 104 Issue: 2 Pages: 128-133
  • DOI: 10.1016/j.ygeno.2014.05.003
  • NAID: 120006763928
  • Peer Reviewed
• [Journal Article] Comparison of the Catalytic Activities of Three Isozymes of Carnitine Palmitoyltransferase 1 Expressed in COS7 Cells (2014)
  • Author(s): Takuya Hada, Takenori Yamamoto, Atsushi Yamamoto, Kazuto Ohkura, Naoshi Yamazaki, Yoshiharu Takiguchi, Yasuo Shinohara
  • Journal Title: Appl Biochem Biotechnol Volume: 172 Issue: 3 Pages: 1486-1496
  • DOI: 10.1007/s12010-013-0619-y
  • Peer Reviewed
• [Presentation] 哺乳類ミトコンドリアのリン酸輸送担体の酵母での機能的発現 (2015)
  • Author(s): 山越亮平、山本武範、寺田弘、篠原康雄
  • Organizer: 第37回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 熊本大学（熊本県熊本市）
  • Year and Date: 2015-11-19
• [Presentation] ミトコンドリアのADP/ATP輸送体に対する ボンクレキン酸誘導体の阻害作用 (2015)
  • Author(s): 山本篤司, 奥田勝博, 安部真人, 松本健司, 山本武範, 大倉一人, 寺田弘, 新藤充, 篠原康雄
  • Organizer: 第43回構造活性相関シンポジウム
  • Place of Presentation: 新潟日報メディアシップ（新潟県新潟市）
  • Year and Date: 2015-09-27
• [Presentation] マイクロアレイで遺伝子発現の定量的評価は可能か (2015)
  • Author(s): 秦 拓也、尾華絵理子、角幡 玲、堀 友繁、山本武範、篠原康雄
  • Organizer: 第 28 回バイオメディカル分析科学シンポジウム
  • Place of Presentation: 長崎大学（長崎県長崎市）
  • Year and Date: 2015-08-21
• [Presentation] デカリニウムはミトコンドリアに透過性遷移を誘起する (2015)
  • Author(s): 島 佐和子, 山本武範, 榎本麻里子, 山下菊治, 寺田 弘, 篠原康雄, 滝口祥令
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸学院大学ほか（兵庫県神戸市）
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] 昆虫ミトコンドリアのADP/ATP carrierの酵母における機能的発現 (2015)
  • Author(s): 原田一樹, 猪谷祐貴, 菅原亮平, 塩月孝博, 三芳秀人, 橋本 満, 真島英司, 寺田 弘, 山本武範, 篠原康雄
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸学院大学ほか（兵庫県神戸市）
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] 抗体導入試薬の性質の違いが細胞内への抗体の導入に及ぼす影響 (2015)
  • Author(s): 桑原かな, 原田一樹, 山越亮平, 山本武範, 篠原康雄
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸学院大学ほか（兵庫県神戸市）
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] プレ配列の削除及び特定のアミノ酸残基の変異による哺乳類のリン酸輸送担体の酵母ミトコンドリアにおける機能的発現 (2015)
  • Author(s): 山越亮平, 山本武範, 篠原康雄
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸学院大学ほか（兵庫県神戸市）
  • Year and Date: 2015-03-25 – 2015-03-28