The crosstalk between growth signals and mitotic signals
Project/Area Number |
25460089
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biological pharmacy
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Research Institution | Aichi Cancer Center Research Institute |
Principal Investigator |
Kasahara Kousuke 愛知県がんセンター(研究所), 腫瘍医化学部, 研究員 (90455535)
|
Research Collaborator |
TANAKA Hiroki 愛知県がんセンター(研究所), 腫瘍医化学部, リサーチレジデント
KAWAMOTO Eriko 愛知県がんセンター(研究所), 腫瘍医化学部, 嘱託技師
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 細胞周期 / 中心体 / リン酸化 / ユビキチン化 / 増殖生存シグナル / 一次繊毛 / 細胞分裂 / 細胞分裂期 / Plk1キナーゼ / PI3キナーゼ / Aktキナーゼ / 14-3-3 |
Outline of Final Research Achievements |
In this study, we have revealed that PI3-kinase-Akt/PKB signaling pathway is essential for proper mitotic progression. In mitosis, Akt kinase activity is responsible for Plk1 (Polo-like kinase 1) phosphorylation at Ser-99. This phosphorylation creates the docking site of 14-3-3 gamma and elevates the Plk1 kinase activity, thereby regulating the metaphase-anaphase transition. We further show that ubiquitin-proteasome system controls primary cilia formation. In response to serum starvation, the ubiquitin E3 ligase CRL3-KCTD17 induces polyubiqutination-dependent degradation of trichoplein, one of the most important negative regulator of ciliogenesis. The trichoplein degradation attenuates the kinase activity of Aurora A, and thereby triggers ciliogenesis.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.2016
Author(s)
Inaba H, Goto H, Kasahara K, Kumamoto K, Yonemura S, Inoko A, Yamano S, Wanibuchi H, He D, Goshima N, Kiyono T, Hirotsune S, Inagaki M.
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Journal Title
J Cell Biol.
Volume: 212
Issue: 4
Pages: 409-423
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] PI 3-kinase-dependent phosphorylation of Plk1-Ser99 promotes association with 14-3-3γ and is required for metaphase-anaphase transition2013
Author(s)
Kasahara, K., Goto, H., Izawa, I., Kiyono, T., Watanabe, N., Elowe, S., Nigg, E.A. and Inagaki, M.
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Journal Title
Nat. Commun.
Volume: 4
Issue: 1
Pages: 1882-1882
DOI
Related Report
Peer Reviewed / Open Access
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