Metabolism of major phytocannabinoids in human brain and lung microsomes and its toxicological implication
| Project/Area Number |
25460176
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | Hokuriku University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 大麻成分 / テトラヒドロカンナビノール / カンナビジオール / カンナビノール / 脳 / 肺 / 代謝 / 細胞毒性 / ヒト脳ミクロソーム / ヒト肺ミクロソーム / ヒト胎盤ミクロソーム / ﾃﾄﾗﾋﾄﾞﾛｶﾝﾅﾋﾞﾉｰﾙ / ｶﾝﾅﾋﾞｼﾞｵｰﾙ / ｶﾝﾅﾋﾞﾉｰﾙ / ｼﾄｸﾛﾑP450 / ﾋﾄ脳ﾐｸﾛｿｰﾑ / ﾋﾄ肺ﾐｸﾛｿｰﾑ / ﾋﾄ胎盤ﾐｸﾛｿｰﾑ / CYP19 / アナンアミド加水分解 / シトクロムP450 / 酸化的代謝 |
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| Outline of Final Research Achievements |
Metabolic interactions of major phytocannabinoids (tetrahydrocannabinol, THC; cannabidiol, CBD; cannabinol, CBN) with microsomes from human brain, lung and placenta were investigated as part of toxicological study of these cannabinoids on extrahepatic tissues and following points were clarified: (1 & 2) metabolism of cannabinoids with human brain and lung microsomes. (3) metabolism of cannabinoids with human placenta microsomes and CYP19. (4) microsomal aldehyde oxygenase (MALDO) activity in human brain and lung microsomes for 11-oxo-THC. (5) inhibitory effects of cannabinoids on hydrolysis of anandamide and 2-arachinoylglycerol with human brain and lung microsomes. (6) Cytotoxicity of THC metabolites to mouse lung macrophage J774-1 cells.
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Report
(4 results)
Research Products
(35 results)
