| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Sense of olfaction starts at the olfactory cilia of the olfactory receptor cells. Two types of olfactory transduction channels expresses on the cilia with high density, named CNG and Cl(Ca) channels. These channels cause current responses, which leads to the signals transmission to the brain. Spatial and temporal distributions of cytoplasmic elements (cAMP, Ca2+) are key factors in this cascade. However, structure of cilia are extremely tiny (φ100nm). This is the one of the reason for delaying electrophysiological study of the olfactory receptor cell. Furthermore, we combined three techniques into one set up to examine the excitatory and inhibitory of transduction channels and dynamics of second messengers in single cilium. Three techniques are here. 1) LMS-ROI. 2) Caged substances and photolysis, 3) Patch-clamp recording in parallel. Therefore, we observed the channel inhibition by certain odorants and molecular kinetics in the cilium in the real time using the patch clamp recording.
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