Investigation of c-ktlow murine hematopoietic stem cells for the role in aged animals
| Project/Area Number |
25460483
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Experimental pathology
|
|---|
| Research Institution | Kansai Medical University |
|---|
Principal Investigator |
SASAKI Yutaka 関西医科大学, 医学部, 准教授 (80425066)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SONODA Yoshiaki 関西医科大学, 医学部, 教授 (60206688)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 造血幹細胞 / G0 / c-kit |
|---|
| Outline of Final Research Achievements |
Hematopoietic stem cells (HSCs) stay long in G0 of cell cycle status. The c-kitlow subfraction of the Lin-Sca-1+c-kit+CD150+CD48-HSCs contains cells in G0 in high frequency. Observation in vivo of integrated BrdU in HSCs suggested that HSCs divided slowly with fluctuating c-kit expression in the time course. The c-kitlow subfraction included cells that did not divide in response to pro-proliferation stimuli. These cells were poor in mitochondrial mass and in activities of reactive oxygen species. DNA microarray analyses indicated that c-kitlow cells differed from c-kithigh cells in the level of cell cycle related gene expression as well as genes for transcription, phosphorylation and metabolism.
|
Report
(4 results)
Research Products
(8 results)
