Development of practical mucosal vaccine for the final host of Echinococcus multilocularis and characterization of host protective reaction induced by reinfection of the parasite in dogs
| Project/Area Number |
25460524
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | Hokkaido Institute of Public Health |
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Principal Investigator |
KOUGUCHI Hirokazu 北海道立衛生研究所, 感染症センター感染症部, 主査 (50435567)
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| Co-Investigator(Kenkyū-buntansha) |
YAMANO Kimiaki 北海道立衛生研究所, 感染症センター感染症部, 主査 (40435566)
YAGI Kinpei 北海道立衛生研究所, 感染症センター感染症部, 部長 (70414323)
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| Co-Investigator(Renkei-kenkyūsha) |
OKU Yuzaburo 鳥取大学, 農学部・獣医学科, 教授 (60133716)
MATSUMOTO Jun 日本大学, 生物資源学部 獣医学科, 准教授 (70296169)
URAGUCHI Koji 北海道立衛生研究所, 感染症センター感染症部, 主幹 (80414324)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 多包条虫 / ワクチン / 犬 / 粘膜免疫 / 遺伝子 / バイオテクノロジー / 蛋白質 / エキノコックス / イヌ / 再感染 / 再感染防御 |
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| Outline of Final Research Achievements |
Experimental Echinococcus multilocularis infection and deworming was repeated 3 or 5 times in 9 dogs at various reinfection schedules. The mean number of worms decreased more than 91% in dogs with repeated infection, compared to first infection controls. To examine whether such worm exclusion was a nonspecific response, 5 dogs were sequentially infected with the parasite four times and subsequently fed freely for 6 months. Even after the 6-month interval, the dogs that were infected five times with the parasite were still able to largely exclude the adult worms. The results suggested that the ability of worm exclusion in dogs that developed a resistance was not rapidly extinct. The observation of the faeces condition and the excretion of hooks in the faeces of repeatedly infected dogs revealed that the exclusion of worms started at the first week after the reinfection, and it continued during the patent period. These findings suggested the possibility of developing a vaccine.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] First detection of Echinococcus multilocularis infection in two species of nonhuman primates raised in a zoo: a fatal case in Cercopithecus diana and a strongly suspected case of spontaneous recovery in Macaca nigra2014
Author(s)
Yamano K, Kouguchi H, Uraguchi K, Mukai T, Shibata C, Yamamoto H, Takaesu N, Ito M, Makino Y, Takiguchi M, Yagi K
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Journal Title
Parasitology International
Volume: 63,
Issue: 4
Pages: 621-626
DOI
Related Report
Peer Reviewed
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[Journal Article] Characterization of a surface glycoprotein from Echinococcus multilocularis and its mucosal vaccine potential in dogs2013
Author(s)
Kouguchi, H., Matsumoto, J., Nakao, R., Yamano, K., Oku, Y., and Yagi, K.
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Journal Title
PLoS ONE
Volume: 8
Issue: 7
Pages: e69821-e69821
DOI
Related Report
Peer Reviewed
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