Establishment of biological dose assessment method using the chromosome analysis in low-dose radiation exposure.
| Project/Area Number |
25460694
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | Fukushima Medical University |
|---|
Principal Investigator |
Sakai Akira 福島県立医科大学, 医学部, 教授 (70284221)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KATAFUCHI Atsushi 福島県立医科大学, 放射線生命科学講座, 助教 (40634910)
OGAWA Kazuei 福島県立医科大学, 循環器血液内科, 教授 (40423800)
YOSHIDA Mitsuaki 弘前大学, 被ばく医療総合研究所, 教授 (60182789)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 低線量被ばく / 二動原体染色体 / 転座型染色体 / 医療被ばく / CT検査 / Giemsa染色 / FISH法 / 生物学的線量評価 |
|---|
| Outline of Final Research Achievements |
We performed dicentric chromosome assays (DCAs) and translocation analyses (chromosome painting) before and after a CT scan to assess effects of low-dose ionizing radiation on chromosomes. DCA was performed by analyzing either 1,000 or 2,000 metaphases using both Giemsa staining and centromere-fluorescence in situ hybridization (Centromere-FISH). Translocation analysis was performed by analyzing 2,000 cell equivalents. DIC formation increased significantly after a CT scanning. A good correlation was observed between the increment of DIC formation after a CT scan determined by analysis of 1,000 metaphases and those by 2,000 metaphases using Centromere-FISH, and between the increment of DIC formation determined by analysis of 2,000 metaphases using Giemsa staining and those by 2,000 metaphases using Centromere-FISH. In contrast to the results of DIC formation, the frequency of translocations detected before and after the CT scan did not differ significantly.
|
Report
(4 results)
Research Products
(6 results)
-
-
[Journal Article] Increase in dicentric chromosome formation after a single CT scan in adults.2015
Author(s)
Abe Y, Miura T, Yoshida MA, Ujiie R, Kurosu Y, Kato N, Katafuchi A, Tsuyama N, Ohba T, Inamasu T, Shishido F, Noji H, Ogawa K, Yokouchi H, Kanazawa K, Ishida T, Muto S, Ohsugi J, Suzuki H, Ishikawa T, Kamiya K, Sakai A.
-
Journal Title
Scinetific Reports
Volume: 9
Issue: 1
Pages: 13882-13882
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Presentation] Analyses of dicentric chromosome formation and chromosomal translocation after a single CT scan in adults2015
Author(s)
Yu Abe, Tomisato Miura, Mitsuaki A Yoshida, Risa Ujiie, Yumiko Kurosu, Nagisa Kato, Atsushi Katafuchi, Naohiro Tsuyama, Takashi Ohba, Tomoko Inamasu, Fumio Shishido, Hideyoshi Noji, Kazuei Ogawa, Hiroshi Yokouchi, Kenya Kanazawa, Takashi Ishida, Jun Ohsugi, Hiroyuki Suzuki, Tetsuo Ishikawa, Kenji Kamiya, Akira Sakai
Organizer
15th International Congress of Radiation Research
Place of Presentation
Kyoto
Year and Date
2015-05-28
Related Report
-
[Presentation] Analyses of dicentric chromosome formation and chromosomal translocation after a single CT scan in adults2015
Author(s)
Yu Abe, Tomisato Miura, Mitsuaki A Yoshida, Risa Ujiie, Yumiko Kurosu, Nagisa Kato, Atsushi Katafuchi, Naohiro Tsuyama, Takashi Ohba, Tomoko Inamasu, Fumio Shishido, Hideyoshi Noji, Kazuei Ogawa, Hiroshi Yokouchi, Kenya Kanazawa, Takashi Ishida, Jun Ohsugi, Hiroyuki Suzuki, Tetsuo Ishikawa, Kenji Kamiya, Akira Sakai
Organizer
ICRR（国際放射線影響学会）
Place of Presentation
京都
Year and Date
2015-05-26
Related Report
Int'l Joint Research
-
-
