| Project/Area Number |
25460694
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
Sakai Akira 福島県立医科大学, 医学部, 教授 (70284221)
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| Co-Investigator(Kenkyū-buntansha) |
KATAFUCHI Atsushi 福島県立医科大学, 放射線生命科学講座, 助教 (40634910)
OGAWA Kazuei 福島県立医科大学, 循環器血液内科, 教授 (40423800)
YOSHIDA Mitsuaki 弘前大学, 被ばく医療総合研究所, 教授 (60182789)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 低線量被ばく / 二動原体染色体 / 転座型染色体 / 医療被ばく / CT検査 / Giemsa染色 / FISH法 / 生物学的線量評価 |
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| Outline of Final Research Achievements |
We performed dicentric chromosome assays (DCAs) and translocation analyses (chromosome painting) before and after a CT scan to assess effects of low-dose ionizing radiation on chromosomes. DCA was performed by analyzing either 1,000 or 2,000 metaphases using both Giemsa staining and centromere-fluorescence in situ hybridization (Centromere-FISH). Translocation analysis was performed by analyzing 2,000 cell equivalents. DIC formation increased significantly after a CT scanning. A good correlation was observed between the increment of DIC formation after a CT scan determined by analysis of 1,000 metaphases and those by 2,000 metaphases using Centromere-FISH, and between the increment of DIC formation determined by analysis of 2,000 metaphases using Giemsa staining and those by 2,000 metaphases using Centromere-FISH. In contrast to the results of DIC formation, the frequency of translocations detected before and after the CT scan did not differ significantly.
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