The role of impaired micro circulation or coagulopathy in chronic liver disease

Research Project

Project/Area Number	25461016
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Gastroenterology
Research Institution	Hyogo Medical University
Principal Investigator	NISHIGUCHI SHUHEI 兵庫医科大学, 医学部, 教授 (10192246)
Co-Investigator(Kenkyū-buntansha)	TSUTSUI HIROKO 兵庫医科大学, 医学部, 教授 (40236914) ENOMOTO HIRAYUKI 兵庫医科大学, 医学部, 准教授 (40449880)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2015: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000) Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000) Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	凝固亢進 / 肝障害 / 肝線維化
Outline of Final Research Achievements	We examined the role of coagulopathy in chronic liver disease (CLD).Blood samples and liver tissues were taken from 15 chronic hepatitis (CH)-B, 27 CHC, 10　autoimmune hepatitis (AIH), and 8 non-alcoholic steatohepatitis (NASH).Von Willebrand factor (vWF) was high 8/15 in CHB, 14/27 in CHC, 6/8 in AIH, was low 4/8 in NASH. Thrombin and Antithrombin 3 complex (TAT) was high 3/15 in CHB, 4/27 in CHC, 3/11 in AIH, 2/8 in NASH. Protein C (PC) and S (PS)　were low in 6/15 and 3/15 in CHB,10/27 and 9/27 in CHC, 2/10 and 1/10 in AIH,0/8 and 0/8 in NASH respectively. But these clotting factors rarely working simultaneously. Liver　samples were analyzed by immunohistochemical staining. Both vWF-Ag and PAI-1 were stained uniformly in the liver tissue, especially around sinusoids. Hypercoagulopathy (high vWF and low PC and PS) was found in CLD, lead to thrombin overproduction in some cases.