| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We extracted mRNA and microRNA from endothelial cells in HCC tissues. The expression of twelve mRNAs related angiogenesis was upregulated. The expression of miR-127-3p, miR-379-5p and miR-711 was upregulated and 70 microRNAs were downregulated in HCC tissues. miR30a-5p which was the most downregulated was added to cultured endothelial cells from HCC tissues. The growth activity of endothelial cells from HCC tissues was suppressed by miR30a-5p addition. In vivo experiments, tumor growth of HCC was suppressed by miR30a-5p addition from mouse tail vein. Tumor angiogenesis was suppressed in HCC tissues by miR30a-5p addition. However, sinusoidal density was not reduced by miR30a-5p.
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