| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Outline of Final Research Achievements |
Previous studies have shown that patients with Alzheimer's disease (AD) exhibit abonormal brain glycolipids compositions and quantities. In this study, we forcused on the integrity and function of the membrane rafts of neuronal cells expressing mutated presenilin-1. We also performed lipid analyses of human brains of AD patients and found decreased amount of neutral glycolipids and gangliosides in the patients compared with those of control subjects. Furthermore, we reconstituted glycolipids abnormality found in patients' brain by use of the specific inhibitors for synthesizing or catabolizing enzymes and/or exogenous addition of glycolipids in culture medium of PC12 cells. During the course of the study, we found novel antibodies against neutral glycolipids. By use of these new autoantibodies against neutral lipids, we examined the specific roles of neutral glycolipids on the function of the rafts in neuronal cells.
|
