| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
(Summary) We performed functional analyses of miR142 using two mouse models. As a result, miR142 overexpression was found to cause a decrease in blood cells. (Result) (1) Bone marrow transplantation model: MiR142 and the GFP genes were introduced into bone marrow cells. The genes-introduced bone marrow cells were transplanted into the bone marrow-disrupted mouse. As a result, the blood cells which strongly express the miR142 were reduced. (2) Genetically modified model: Genetically modified mouse to be strongly expressed in B cells was generated. In genetically modified mice that were older than one year of age, IgM-positive cells were reduced compared to the wild type. (Conclusion) As mentioned above, our results suggest that miR142 overexpression is involved in the cancer development through disruption of cell fate.
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