Clinical importance of anti interferon-gamma autoantibodies

• Project/Area Number: 25461507
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Niigata University
• Principal Investigator: Hasegawa Takashi 新潟大学, 医歯学総合病院, 准教授 (90361906)
• Co-Investigator(Kenkyū-buntansha): SAKAGAMI TAKURO 新潟大学, 医歯学系, 助教 (00444159) ; TANABE YOSHINARI 新潟大学, 医歯学総合病院, 准教授 (40444161)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 播種性非結核性抗酸菌症 / 抗インターフェロンガンマ抗体 / 中和能 / 日和見感染症 / 抗インターフェロンガンマ中和抗体 / 非結核性抗酸菌症 / Jurakat細胞 / フローサイトメトリー / ELISA

Outline of Final Research Achievements

In the recent years, we have an increasing trend non- tuberculous mycobacterial disease around the world. Anti-interferon gamma autoantibodies were often found in formerly healthy patients with disseminated NTM infections. In this study we intend to establish the measurement method of the autoantibodies in clinical specimens from patients.
We have developed the method to evaluate the neutralizing capacity to interferon-gamma of sera and to quantify the relative concentrations of the autoantibodies. Twenty-six antibody-positive cases were observed so far and also clarified their clinical characteristics. Antibody titer in response to the disease of non- tuberculous mycobacterial disease tend to increase or decrease , it is also useful as a marker to reflect the disease has been suggested .

Research Products

• [Journal Article] Disseminated Mycobacterium gordonae and Mycobacterium mantenii infection with elevated anti-IFN-γ neutralizing autoantibodies. (2015)
  • Author(s): Hase I, Morimoto K, Sakagami T, Kazumi Y, Ishii Y, van Ingen J
  • Journal Title: J Infect Chemother. Volume: 21 Issue: 6 Pages: 468-72
  • DOI: 10.1016/j.jiac.2015.02.003
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Recurrence of Disseminated Mycobacterium avium Complex Disease in a Patient with Anti-Gamma Interferon Autoantibodies by Reinfection. (2015)
  • Author(s): Nishimura T, Fujita-Suzuki Y, Yonemaru M, Ohkusu K, Sakagami T, Carpenter SM, Otsuka Y, Namkoong H, Yano I, Hasegawa N
  • Journal Title: J Clin Microbiol Volume: 53 Issue: 4 Pages: 1436-1438
  • DOI: 10.1128/jcm.03339-14
  • Peer Reviewed / Open Access
• [Journal Article] 抗Interferon-γ中和自己抗体陽性の播種性非結核性抗酸菌症 宿主要因からの新たな疾患概念 (2015)
  • Author(s): 坂上拓郎
  • Journal Title: 結核 Volume: 90 Pages: 561-564
  • Peer Reviewed
• [Journal Article] Novel assay to detect increased level of neutralizing anti-interferon gamma autoantibodies in non-tuberculous mycobacterial patients. (2014)
  • Author(s): Shima K, Sakagami T, Tanabe Y, Aoki N, Moro H, Koya T, Kagamu H, Hasegawa T, Suzuki E, Narita I.
  • Journal Title: J Infect Chemother Volume: 20 Issue: 1 Pages: 52-56
  • DOI: 10.1016/j.jiac.2013.08.003
  • Peer Reviewed
• [Journal Article] 非結核性抗酸菌感染症と抗Interferon‐γ自己抗体 (2013)
  • Author(s): 島賢治郎、坂上拓郎、田邊嘉也
  • Journal Title: 感染・炎症・免疫 Volume: 43 Pages: 71-73
• [Presentation] 肺非結核性抗酸菌症、肺結核症における抗Interferon-γ自己抗体に関する検討 (2015)
  • Author(s): 青木 亜美, 坂上 拓郎, 島 賢治郎, 青木 信将, 茂呂 寛, 田邊 嘉也, 小屋 俊之, 各務 博, 成田 一衛, 星野 仁彦, 森本 耕三, 倉島 繁行
  • Organizer: 第55回日本呼吸器学会学術講演会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2015-04-17
• [Presentation] 抗Interferon-γ中和自己抗体陽性の播種性非結核性抗酸菌症の検討 (2014)
  • Author(s): 坂上拓郎、島賢治郎、田邊嘉也、青木信将、茂呂寛、長谷川隆志、成田一衛
  • Organizer: 第88回日本感染症学会学術講演会
  • Place of Presentation: ヒルトン福岡シーホーク
  • Year and Date: 2014-06-18 – 2014-06-20
• [Presentation] Characteristic Phenotypes of Disseminated Non-Tuberculous Mycobacterium Infection with Anti Interferon-γ Autoantibodies in Japan (2014)
  • Author(s): T. Sakagami, K. Shima, Y. Tanabe, N. Aoki, H. Moro, T. Koya ,T. Hasegawa, H. Kagamu, I.Narita
  • Organizer: ATS 2014 International Conference
  • Place of Presentation: San Diego Convention Center
  • Year and Date: 2014-05-16 – 2014-05-21
• [Presentation] 抗Interferon-γ中和自己抗体陽性の播種性非結核性抗酸菌症 ～宿主要因からの新たな疾患概念～ (2014)
  • Author(s): 坂上拓郎
  • Organizer: 第89回日本結核病学会総会
  • Place of Presentation: 長良川国際会議場
  • Year and Date: 2014-05-09 – 2014-05-10
  • Invited
• [Presentation] 抗Interferon-γ中和自己抗体陽性の播種性非結核性抗酸菌症 ～新たな疾患概念～ (2014)
  • Author(s): 島 賢治郎、坂上拓郎、青木信将、茂呂 寛、田邊嘉也、各務 博、長谷川隆志、高田俊範、成田一衛
  • Organizer: 第111回日本内科学会総会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2014-04-11 – 2014-04-13
• [Presentation] 播種性非結核性抗酸菌症における抗IFNγ自己抗体検出法の確立 (2013)
  • Author(s): 島賢治郎，坂上拓郎，青木信将，茂呂寛，田邊嘉也，鈴木栄一，成田一衛，桑原克弘
  • Organizer: 第53回日本呼吸器学会学術講演会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] A Novel Assay to Detect Neutralizing Anti-Interferon-Gamma Autoantibody in Clinical Samples with Disseminated Non-Tuberculous Mycobacterial Infection (2013)
  • Author(s): Shima K, Sakagami T, Aoki N, Moro H, Tanabe Y, Suzuki E, Kuwabara K, Narita I
  • Organizer: American Thoracic Society International Conference 2013
  • Place of Presentation: Pennsylvania Convention Center, philadelphia, USA
• [Presentation] A Novel Assay to Detect Neutralizing Anti-Interferon-Gamma Autoantibody in Clinical Samples with Disseminated Non-Tuberculous Mycobacterial Infection (2013)
  • Author(s): Shima K, Sakagami T, Aoki N, Moro H, Tanabe Y, Koya T, Hasegawa T, Suzuki E, Narita I
  • Organizer: 18th Congress of the APSR
  • Place of Presentation: The Conference Center, Pacifico Yokohama