Development of the diagnostic marker in mucormycosis

• Project/Area Number: 25461516
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Osaka City University
• Principal Investigator: Kakeya Hiroshi 大阪市立大学, 大学院医学研究科, 教授 (40398152)
• Co-Investigator(Kenkyū-buntansha): MIYAZAKI Yoshitsugu 国立感染症研究所, 真菌部, 部長 (00311861) ; SHIBUYA Kazutoshi 東邦大学, 医学部, 教授 (20196447) ; KOHNO Shigeru 長崎大学, 医歯薬学総合研究科, 客員研究員 (80136647)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ムーコル症 / 早期診断 / 血清診断 / 接合菌症 / シグナルシークエンストラップ法 / 患者血清

Outline of Final Research Achievements

The diagnosis of mucormycosis was made by the identification of organism in the histopathology with culture confirmation. However, culture often yields no growth, and histopathological identification of organism with typical of Mucorales is sometimes difficult. Therefore, the reliable new diagnostic tool is expected. Secreted and cell-surface molecules are as target antigens for diagnostic tools. The signal sequence trap (SST) is a strategy to identify signal sequence containing cDNAs that encode secreted and cell-surface proteins. We constructed a SST c DNA library from RNA extraction from the clinical isolate Rhizopus oryzae. The ELISA kit to detect the target antigen was made and evaluated. The antigen A was also detected in R. oryzae infected mice sera. The 23kDa of the hypothetical protein, Antigen A, is a potential novel diagnostic maker in mucormycosis.

Research Products

• [Journal Article] Clinical feature of pulmonary cryptococcosis in non-HIV patients in Japan. (2015)
  • Author(s): Kohno S, Kakeya H, Izumikawa, Miyazaki T, Yamamoto Y, Yanagihara K, Mitsutake K, Miyazaki Y, Maesaki S, Yasuoka A, Tashiro T, Mine M, Uetani M, Ashizawa K.
  • Journal Title: Journal of Infection and Chemotherapy Volume: 21 Pages: 23-30
  • NAID: 120006986896
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Efficacy of combination with oseltamivir phosphate and azithromycin for influenza: a multicenter, open-label, randomized study. (2014)
  • Author(s): Kakeya H, Seki M, Izumikawa K, Kosai K, Morinaga Y, Kurihara S, Nakamura S, Imamura Y, Miyazaki T, Tsukamoto M, Yanagihara K, Tashiro T, Kohno S.
  • Journal Title: PLoS One. Volume: 14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Three Cases of Concurrent Infection With Mycobacterium tuberculosis and Cryptococcus neoformans. (2014)
  • Author(s): Kakeya H, Izumikawa K, Yamada K, Obata Y, Nishino T, Takazono T, Kosai K, Kurihara S, Nakamura S, Imaura Y, Miyazaki T, Tsukamoto M, Yanagihara K, Tashiro T, Kohno S
  • Journal Title: Internal Medicine Volume: 53 Pages: 1685-1692
  • NAID: 130004678360
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Concurrent subcutaneous Candidal abscess and pulmonary Cryptococcosis in patient with diabetes mellitus and a history of corticosteroid therapy. (2014)
  • Author(s): Kakeya H, Izumikawa K, Yamada K, Narita Y, Nishino T, Obata Y, Takazono T, Kuraihara S, Kosai K, Morinaga Y, Nakamura S, Imamura Y, Miyazaki T, Tsukamoto M, Tsukamoto M, Yanagihara K, Takenaka M, Tashiro T, Kohono S.
  • Journal Title: Internal Medicine Volume: 53 Pages: 1385-1390
  • NAID: 130004466351
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Three Cases of Concurrent Infection With Mycobacterium tuberculosis and Cryptococcus neoformans. (2014)
  • Author(s): Kakeya H, Izumikawa K, Yamada K, Obata Y, Nishino T, Takazono T, Kosai K, Kurihara S, Nakamura S, Imaura Y, Miyazaki T, Tsukamoto M, Yanagihara K, Tashiro T, Kohno S
  • Journal Title: Intern Med. Volume: Accept済
  • NAID: 130004678360
  • Peer Reviewed
• [Journal Article] Concurrent subcutaneous Candidal abscess and pulmonary Cryptococcosis in patient with diabetes mellitus and a history of corticosteroid therapy (2014)
  • Author(s): Kakeya H, Izumikawa K, Yamada K, Narita Y, Nishino T, Obata Y, Takazono T, Kuraihara S, Kosai K, Morinaga Y, Nakamura S, Imamura Y, Miyazaki T, Tsukamoto M, Tsukamoto M, Yanagihara K, Takenaka M, Tashiro T, Kohono S
  • Journal Title: Intern Med. Volume: Accept済
  • NAID: 130004466351
  • Peer Reviewed
• [Presentation] Identification of 23kda of Antigen as a Candidate of the Diagnostic Marker in Mucormycosis by Rhizopus oryzae. (2015)
  • Author(s): Kakeya H, Miyazaki Y, Yamagoe S, Sato K, Yamada K, Niki M, Kaneko Y, Kohno S
  • Organizer: ICAAC/ICC 2015
  • Place of Presentation: San Diego, USA
  • Year and Date: 2015-09-17
  • Int'l Joint Research