| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Pluripotent embryonic stem (ES) cells may differentiate into neurons in vitro. It has been shown that astrocyte-derived factors promote mouse ES cells to differentiate into neurons. Cultured in astrocyte-conditioned medium (ACM) under free-floating conditions, within 4 days, colonies of undifferentiated mouse ES cells give rise to floating spheres of concentric stratiform structure with a periphery of neural stem cells, which are termed NSS. Culturing the spheres on an adhesive substrate in ACM promotes neurogenesis, and cells in the spheres differentiate intoneurons within 5 days . The mechanism was examined how neural stem cells (NSCs) transplanted to the hippocampus of EL brains differentiate into inhibitory neurons and play a role of preventing seizures. that brain regions involved in seizure formation, such as hippocampus and parietal cortex in EL mice, have a potency to control seizures by inducing inhibitory GAD neurons.
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