Availability of combined PCC and PNA-FISH method to cytogenetic biodosimetry

• Project/Area Number: 25461869
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Hirosaki University
• Principal Investigator: Yoshida Mitsuaki 弘前大学, 被ばく医療総合研究所, 教授 (60182789)
• Co-Investigator(Kenkyū-buntansha): Miura Tomisato 弘前大学, 保健学研究科, 准教授 (20261456) ; Kasai Kosuke 弘前大学, 保健学研究科, 講師 (50400148) ; Nakata Akifumi 北海道薬科大学, 薬学部, 講師 (70415420)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 染色体線量評価 / PCC法 / Dic法 / FISH法 / 高線量被ばく / PCC-ring法 / 二動原体法 / PCC-dic法 / 二動原体染色体 / セントロメアFISH

Outline of Final Research Achievements

In order to determine the treatment condition for premature chromosome condensation, we investigated the PCC frequency, cell cycle stage and the length of chromosome #2 in normal and irradiated lymphocytes from healthy donor. We found that treatment by 50nM Calyculin A for 30 min. is available for the analysis of chromosome abnormalities including both ring and dicentric chromosomes. In the present study, we also analyzed the availability of staining methods; PCC-ring, PCC- FISH, DCA- FISH and Giemsa staining for the chromosome analysis to establish the method for cytogenetic biodosimetry covering the range from low (1-5Gy) to high (over 5Gy to 25Gy) dose radiation. The results indicates that the frequencies of dicentric chromosome are higher in PCC-centromere FISH and DCA-centromere FISH than Giemsa staining method. Furthermore, it will be possible to establish the standard curve used in the wide range of radiation to estimate radiation dose in the radiological accident.

Research Products

• [Journal Article] Increase in dicentric chromosome formation after a single CT scan in adults. (2015)
  • Author(s): Abe Y, Miura T, Yoshida MA, Ujiie R, Kurosu Y, Kato N, Katafuchi A, Tsuyama N, Ohba T, Inamasu T, Shishido F, Noji H, Ogawa K, Yokouchi H, Kanazawa K, Ishida T, Muto S, Ohsugi J, Suzuki H, Ishikawa T, Kamiya K, Sakai A.
  • Journal Title: Scinetific Reports Volume: 9 Issue: 1 Pages: 13882-13882
  • DOI: 10.1038/srep13882
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of low-dose responsive metabolites in X-irradiated human B lymphoblastoid cells and fibroblasts (2015)
  • Author(s): Tsuyama N, Mizuno H, Katafuchi A, Abe Y, Kurosu Y, Yoshida M, Kamiya K, Sakai A
  • Journal Title: Journal of Radiation Research Volume: 56 Pages: 46-58
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Fetal irradiation of rats induces persistent translocations in mammary epithelial cells similar to the level after adult irradiation, but not in hematolymphoid cells (2014)
  • Author(s): Nakano M, Nishimura M, Hamasaki K, Mishima S, Yoshida M, Nakata A, Shimada Y, Noda A, Nakamura N, Kodama Y.
  • Journal Title: Radiation Research Volume: 181 Pages: 172-176
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Internet's role in a biodosimetric response to a radiation mass casualty event (2014)
  • Author(s): Sugarman SL, Livingston GK, Stricklin DL, Abbott MG, Wilkins RC, Romm H, Oestreicher U, Yoshida MA, Miura T, Moquet JE,Di Giorgio M, Ferrarotto C, Gross GA, Christiansen ME, Hart CL, Christensen DM
  • Journal Title: Health Physics Volume: 106
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A novel parameter, cell-cycle progression index, for radiation dose absorbed estimation in the premature chromosome condensation assay (2014)
  • Author(s): Miura T, Nakata A, Kasai K, Nakano M, Abe Y, Tsushima E, Ossetrova NI, Yoshida MA, Blakely WF
  • Journal Title: Radiation Protection Dosimetry Volume: 159 Pages: 52-60
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] High-incidence spontaneous tumors in JF1/Ms mice: relevance of hypomorphic germline mutation and subsequent promoter methylation of Ednrb. (2014)
  • Author(s): Junko Watanabe, Yasuhiko Kaneko,Masafumi Kurosumi, Yasuhito, Kobayashi, Michihiro Sakamoto, Mitsuaki A. Yoshida, Miho Akiyama, Yoshibumi Matsushima
  • Journal Title: J Cancer Res Clin Oncol Volume: 140 Pages: 99-107
  • Peer Reviewed
• [Journal Article] Activity concentrations of environmental samples collected in Fukushima Prefecture immediately after the Fukushima nuclear accident. (2013)
  • Author(s): Hosoda M, Tokonami S, Tazoe H, Sorimachi A, Monzen S, Osanai M, Akata N, Kakiuchi H, Omori Y, Ishikawa T, Sahoo SK, Kovács T, Yamada M, Nakata A, Yoshida M, Yoshino H, Mariya Y, Kashiwakura I
  • Journal Title: Sci. Rep Volume: 3 Pages: 2283-2283
  • Peer Reviewed
• [Journal Article] Healthcare of 18 Workers who Supported the Regulation of Radiological Contaminations at the Fukushima Daiichi Nuclear Power Plant (2013)
  • Author(s): Miura T, Nakata A, Kasai K, Abe Y, Jin Y, Yoshida MA
  • Journal Title: Radiation Emergency Medicine Volume: 2 Pages: 63-67
  • NAID: 40019745115
  • Peer Reviewed
• [Journal Article] Thyrpoid Screening survey on Children after the Fukushima Daiichi Nuclear Power Plant accident. (2013)
  • Author(s): Yoichiro Hosokawa, Masahiro Hosoda, Akifumi Nakata, Masanori Kon, Mayumi Urusizaka and Mitsuaki A. Yoshida
  • Journal Title: Radiation Emergency Medicine Volume: 2 Pages: 82-86
  • Peer Reviewed
• [Presentation] Comparison of the cell-cycle progression index and ring chromosome for dose estimation using simulated human peripheral blood partial-body irradiation in the premature chromosome condensation assay (2015)
  • Author(s): Miura T, Obayashi A, Yamaguchi Y, Tsutsumi Y, Akitaya Y, Abe Y, Fujishima Y, Nakata A, Ariyoshi K, Yoshida MA.
  • Organizer: EPR BioDose2015
  • Place of Presentation: Hanover, New Hampshire USA
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Presentation] Analyses of dicentric chromosome and chromosomal translocation after a single CT scan in adults (2015)
  • Author(s): Abe Y, Miura T, Yoshida MA, Ujiie R, Kurosu Y, Kato N, Katafuchi A, Tsuyama N, Ohba T, Inamasu T, Shishido F, Noji H, Ogawa K, Yokouchi H, Kanazawa K, Ishida T, Muto S, Ohsugi J, Suzuki H, Ishikawa T, Kamiya K, Sakai A.
  • Organizer: EPR BioDose2015
  • Place of Presentation: Hanover, New Hampshire USA
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Presentation] Investigation of the Radioprotective Effect of Tomato Juice Containing Lycopene in Healthy Donors (2015)
  • Author(s): Nakamura A, Fujishima Y, Fukushi Y, Kitajima M, Monzen A, Miura T, Saito A, Yonezawa T, Suganuma H, Aizawa K, Itaki C, Yoshida MA, Wojcik A, Mariya Y, Haghdoost S.
  • Organizer: The 15th International Congress of Radiation Research (ICRR 2015)
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2015-05-25
  • Int'l Joint Research
• [Presentation] Assessment of Simulated Partial Body Acute Irradiation by Cell-cycle Progression Index in the Premature Chromosome Condensation Assay (2015)
  • Author(s): Miura T, Abe Y, Nakata A, Kasai K, Yoshida MA.
  • Organizer: The 15th International Congress of Radiation Research (ICRR 2015)
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2015-05-25
  • Int'l Joint Research
• [Presentation] Effects of Radioactive Pollution of the Japanese Large Field Mouse (Apodemus specious) in Fukushima (2015)
  • Author(s): Nakata A, Fujishima Y, Ujiie R, Kasai K, Tazoe H, Ariyoshi K, Saito M, Suzuki K, Yamada M, Miura T, Yoshida MA, Fukumoto M.
  • Organizer: The 15th International Congress of Radiation Research (ICRR 2015)
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2015-05-25
  • Int'l Joint Research
• [Presentation] Radiation Dosimetry for The Internal Exposure of The Cats in Namie-town (2015)
  • Author(s): Fujishima Y, Nakata A, Miura T, Tazoe H, Toyoda T, Kasai K, Ariyoshi K, Yamada M, Konno N, Yoshida MA
  • Organizer: 2nd. Educational Symposium on Radiation and Health by young scientists (ESRAH) 2015
  • Place of Presentation: Hirosaki University, Hirosaki, Japan
  • Year and Date: 2015-05-23
  • Int'l Joint Research
• [Presentation] Current status of cytogenetic biodosemetry in radiation emergency medicine (2014)
  • Author(s): M. Yoshida, K. Ariyoshi
  • Organizer: FNCA Workshop on Radiation Oncology Open lecture
  • Place of Presentation: Hirosaki, Aomori, Japan
  • Year and Date: 2014-11-05
  • Invited
• [Presentation] 放射性物質汚染地域に生息するアカネズミの生物影響解析 (2014)
  • Author(s): 三浦富智、中田章史、藤嶋洋平、氏家里紗、葛西宏介、田副博文、有吉健太郎、齋藤幹男、鈴樹亨純、山田正俊、吉田光明、福本学
  • Organizer: 日本放射線影響学会第57回大会
  • Place of Presentation: 鹿児島市
  • Year and Date: 2014-10-01 – 2014-10-03
• [Presentation] 炭素線で誘発させたマウス胸腺リンパ腫の仔マウスにおける染色体異常の特徴 (2014)
  • Author(s): 吉田光明、中田章史、藤嶋洋平、高橋千佳、外舘茜、有吉健太郎、柿沼志津子、島田 義也
  • Organizer: 日本放射線影響学会第57回大会
  • Place of Presentation: 鹿児島市
  • Year and Date: 2014-10-01 – 2014-10-03
• [Presentation] 染色体解析による福島県浪江町被災住民の初期被ばく検査 (2014)
  • Author(s): 吉田光明，中田章史，三浦富智，西村美八，高間木静香，葛西宏介、紺野則夫，吉田良子，関根俊二
  • Organizer: 日本学術会議・日本衛生学会公開シンポジウム
  • Place of Presentation: 岡山市
  • Year and Date: 2014-05-25
  • Invited
• [Presentation] Construction of chromosome translocation analysis system for biodosimetry in the children of Namie-Town after the Fukushima accident (2014)
  • Author(s): Mitsuaki A. Yoshida, Akifumi Nakata, Tomisato Miura, Miya Nishimura, Shizuka Takamagi, Kosuke Kasai, Norio Konno, Ryoko Yoshida and Shunji Sekine
  • Organizer: 14th WHO REMPAN Meeting
  • Place of Presentation: Wurzburg, Germany
  • Year and Date: 2014-05-07 – 2014-05-09
• [Presentation] Premature Chromosome Condensation (PCC) analysis (2013)
  • Author(s): Mitsuaki Yoshida
  • Organizer: BIODOSIMETRY IN THE 21st CENTURY Training Meeting HICARE in collaboration with the International Atomic Energy Agency
  • Place of Presentation: Hiroshima
  • Invited
• [Presentation] 福島県浪江町の被災住民における染色体転座解析のためのシステム構築 (2013)
  • Author(s): 中田章史，吉田光明，三浦富智，西村美八，高間木静香，葛西宏介，紺野則夫，吉田良子，関根俊二
  • Organizer: 日本放射線影響学会第５６回大会
  • Place of Presentation: 青森市
• [Presentation] 放射性物質汚染地域におけるアカネズミの個体群調査 (2013)
  • Author(s): 吉田光明，藤嶋洋平，氏家里紗，葛西宏介，三浦富智，対馬栄輝，鈴樹亨純，中田章史
  • Organizer: 日本放射線影響学会第５６回大会
  • Place of Presentation: 青森市
• [Presentation] 土壌中の放射能比分析による福島第一原子力発電所事故由来のプルトニウムの飛散状況の把握 (2013)
  • Author(s): 山口朋子、山田正俊、田副博文、葛西宏介、中田章史、細川洋一郎
  • Organizer: 平成２５年度青森県放射線技師学術大会
  • Place of Presentation: 弘前市