| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
This study aimed the development ofhigh sensitive detection method for CTC/micrometastasesand its preclinical and clinical application to evaluate tumor malignancy and chemosensitivity for the neoadjuvant chemotherapy. We newly developed a filtration-based, sensitive CTC isolation device and mouse CTC model. Using this system, we demonstrated M stage-arrested CTC in mouse blood after Paclitaxel treatment, indicatingthe possibility for successful evaluation of chemosensitivity at an early stage after therapy. We also revealed that our CTC isolation device allows more sensitive CTC detection and more rapid genetic analysis of patient’s blood samples than the conventional device. These results suggest that this CTC isolation device would be an useful tool to evaluate tumor malignancy and chemosensitivityas a liquid biopsy.
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