Development of novel microRNA-based therapy against KRAS/BRAF-mutant colorectal cancer
Project/Area Number |
25462057
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Digestive surgery
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Research Institution | Okayama University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | マイクロRNA / 大腸癌 / KRAS / BRAF / EGFR |
Outline of Final Research Achievements |
KRAS/BRAF-mutant colorectal cancer is an intractable disease that frequently shows the refractory to chemotherapy, recurrence and metastatic progression. Since EGFR signaling pathway is constitutively activated in KRAS/BRAF-mutant colorectal cancer, the development of novel therapy for strongly suppressing EGFR signaling pathway is important issue. Here we show that oncolytic adenoviruses have profound antitumor effect through activation of EGFR-suppressive microRNA and induction of apoptosis- and autophagy-related cell death against KRAS/BRAF-mutant colorectal cancer.
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Report
(3 results)
Research Products
(22 results)
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[Journal Article] Biological ablation of sentinel lymph node metastasis in submucosally invaded early gastrointestinal cancer.2015
Author(s)
Kikuchi S, Kishimoto H, Tazawa H, Hashimoto Y, Kuroda S, Nishizaki M, Nagasaka T, Shirakawa Y, Kagawa S, Urata Y, Hoffman RM, Fujiwara T.
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Journal Title
Mol Ther.
Volume: 23(3)
Issue: 4
Pages: 501-509
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Viral transduction of the HER2-extracellular domain expands trastuzumab-based photoimmunotherapy for HER2-negative breast cancer cells2015
Author(s)
Shimoyama K, Kagawa S, Ishida M, Watanabe S, Noma K, Takehara K, Tazawa H, Hashimoto Y, Tanabe S, Matsuoka J, Kobayashi H, Fujiwara T
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Journal Title
Breast Cancer Reseach and Treatment
Volume: 149
Issue: 3
Pages: 597-605
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Cancer cells mimic in vivo spatial-temporal cell-cycle phase distribution and chemosensitivity in 3-dimensional gelfoam histoculture but not 2-dimensional culture as visualized with real-time FUCCI imaging2015
Author(s)
Yano S, Miwa S, Mii S, Hiroshima Y, Uehara F, Kishimoto H, Tazawa H, Zhao M, Bouvet M, Fujiwara T, Hoffman RM
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Journal Title
Cell Cycle
Volume: 14
Issue: 6
Pages: 808-819
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Experimental curative fluorescence-guided surgery of highly-invasive glioblastoma multiforme selectively labeled with a killer-reporter adenovirus2015
Author(s)
Yano S, Miwa S, Kishimoto H, Toneri M, Hiroshima Y, Yamamoto M, Bouvet M, Urata Y, Tazawa H, Kagawa S, Fujiwara T, Hoffman RM
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Journal Title
Mol Ther
Volume: 23
Issue: 7
Pages: 1182-1188
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Targeting tumors with a killer-reporter adenovirus for curative fluorescence-guided surgery of soft-tissue sarcoma2015
Author(s)
Yano S, Miwa S, Kishimoto H, Uehara F, Tazawa H, Toneri M, Hiroshima Y, Yamamoto M, Urata Y, Kagawa S, Bouvet M, Fujiwara T
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Journal Title
Oncotarget
Volume: 6
Pages: 13133-13148
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Color-coding cancer and stromal cells with genetic reporters in a patient-derived orthotopic xenograft (PDOX) model of pancreatic cancer enhances fluorescence-guided surgery.2015
Author(s)
Yano S, Hiroshima Y, Maawy A, Kishimoto H, Suetsugu A, Miwa S, Toneri M, Yamamoto M, Katz MH, Fleming JB, Urata Y, Tazawa H, Kagawa S, Bouvet M, Fujiwara T, Hoffman RM.
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Journal Title
Cancer Gene Ther
Volume: 22
Issue: 7
Pages: 344-50
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Genetic and epigenetic alterations of netrin-1 receptors in gastric cancer with chromosomal instability.2015
Author(s)
Toda K, Nagasaka T, Umeda Y, Tanaka T, Kawai T, Fuji T, Taniguchi F, Yasui K, Kubota N, Takehara Y, Tazawa H, Kagawa S, Sun DS, Nishida N, Goel A, Fujiwara T.
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Journal Title
Clin Epigenetics
Volume: 7
Issue: 1
Pages: 73-73
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Invading cancer cells are predominantly in G0/G1 resulting in chemoresistance demonstrated by real-time FUCCI imaging2014
Author(s)
Yano S, Miwa S, Mii S, Hiroshima Y, Uehara F, Yamamoto M, Kishimoto H, Tazawa H, Bouvet M, Fujiwara T, Hoffman RM
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Journal Title
Cell Cycle
Volume: 13
Issue: 6
Pages: 953-960
DOI
Related Report
Peer Reviewed
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[Journal Article] Chronic inflammation-derived nitric oxide causes conversion of human colonic adenoma cells into adenocarcinoma cells2013
Author(s)
Tazawa H, Kawaguchi T, Kobayashi T, Kuramitsu Y, Wada S, Satomi Y, Nishino H, Kobayashi M, Kanda Y, Osaki M, Kitagawa T, Hosokawa M and Okada F
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Journal Title
Exp Cell Res
Volume: 319
Issue: 18
Pages: 2835-2844
DOI
Related Report
Peer Reviewed
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[Journal Article] A genetically engineered oncolytic adenovirus decoys and lethally traps quiescent cancer stem-like cells into S/G2/M-phases.2013
Author(s)
Yano S, Tazawa H, Hashimoto Y, Shirakawa Y, Kuroda S, Nishizaki M, Kishimoto H, Uno F, Nagasaka T, Urata Y, Kagawa S, Hoffman RM, Fujiwara T
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Journal Title
Clin Cancer Res
Volume: 19
Issue: 23
Pages: 6495-6505
DOI
Related Report
Peer Reviewed
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[Presentation] Preclinical evaluation of tumor-specific oncolytic virotherapy against human colorectal cancer cells with different KRAS/BRAF mutation and microsatellite stability2015
Author(s)
Tamura S, Tazawa H, Hori N, Koujima T, Kikuchi S, Kuroda S, Kishimoto H, Nagasaka T, Nishizaki M, Urata Y, Kagawa S, Fujiwara T
Organizer
第21回日本遺伝子治療学会
Place of Presentation
大阪
Year and Date
2015-07-24
Related Report
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[Presentation] Companion diagnostics-based telomerase-specific oncolytic virotherapy: preclinical evaluation in human colorectal cancer cell lines differentially affected in the RAS/RAF/MEK signaling pathway2015
Author(s)
Tamura S, Tazawa H, Hori N, Koujima T, Kikuchi S, Kuroda S, Kishimoto H, Nagasaka T, Nishizaki M, Urata Y, Kagawa S, Fujiwara T
Organizer
106th Annual Meeting of the American Association for Cancer Research
Place of Presentation
Philadelphia, USA
Year and Date
2015-04-18
Related Report
Int'l Joint Research
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[Presentation] A novel apoptotic mechanism of genetically engineered adenovirus-mediated tumor-specific p53 overexpression through E1A-dependent p21 and MDM2 suppression2013
Author(s)
Tazawa H, Yamasaki Y, Hashimoto Y, Kuroda S, Yano S, Yoshida R, Uno F, Mizuguchi H, Ohtsuru A, Urata Y, Kagawa S, Fujiwara T
Organizer
第19回日本遺伝子治療学会
Place of Presentation
岡山
Related Report
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