Development of a sensitive system for capturing circulating tumor cells in lung cancer.

• Project/Area Number: 25462191
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory surgery
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: SO Tomoko 産業医科大学, 医学部, 講師 (00341529)
• Co-Investigator(Kenkyū-buntansha): TANAKA Fumihiro 産業医科大学, 医学部, 教授 (10283673) ; URAMOTO Hidetaka 産業医科大学, 医学部, 准教授 (90389445) ; CHIKAISHI Yasuhiro 産業医科大学, 医学部, 助教 (25861251) ; YONEDA Kazue 産業医科大学, 医学部, 助教 (80724806) ; 近石 泰弘 産業医科大学, 医学部, 助教 (70609221)
• Research Collaborator: KATAYORI Satoshi JSRライフサイエンス株式会社, 研究開発部
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 肺癌 / 循環腫瘍細胞 / EpCAM / 細胞分離 / 細胞分離用磁性粒子 / 上皮間葉系移行(EMT) / 悪性胸膜中皮腫

Outline of Final Research Achievements

The “CellSearch” is widely used to detect circulating tumor cells (CTCs), but provided only modest sensitivity in capturing CTCs in lung cancer patients. In the current study, we employed a variety of CTC-detection systems including a novel microfluidic device (“CTC-chip”) in which any antibody to capture CTCs is easily conjugated. In experimental studies using tumor cell lines, the “CTC-chip” provided a similar efficiency to the “CellSearch” in capturing PC-9 cells with EpCAM expression. When MESO-4 cells without EpCAM expression were spiked in the blood, approximately 80% of tumor cells were captured with the “CTC-chip”, whereas few tumor cells were captured with the “CellSearch”.

Research Products

• [Journal Article] Adjuvant therapy following surgery in non-small cell lung cancer (NSCLC). (2016)
  • Author(s): Tanaka F, Yoneda K.
  • Journal Title: Surg Today. Volume: 46（1） Issue: 1 Pages: 25-37
  • DOI: 10.1007/s00595-015-1174-7
  • Peer Reviewed
• [Presentation] Improved efficacy in capturing EpCAM-negative tumor cells using a “Universal CTC-chip” (2015)
  • Author(s): Kazue Yoneda, Yasuhiro Chikaishi, Eri Kawashima, Takashi Ohnaga, Fumihiro Tanaka
  • Organizer: AACR Tumor metastasis
  • Place of Presentation: Austin（USA)
  • Year and Date: 2015-11-30
  • Int'l Joint Research
• [Presentation] 胸部悪性腫瘍におけるCell-based Liquid Biopsy (2015)
  • Author(s): 米田和恵
  • Organizer: 第75回日本呼吸器学会・日本結核病学会・日本サルコイドーシス/肉芽腫性疾患学会 九州支部秋季学術集会
  • Place of Presentation: ホテルグランデはがくれ（佐賀県佐賀市）
  • Year and Date: 2015-10-02
  • Invited
• [Presentation] “Universal CTC-Chip”によるEpCAM陰性循環腫瘍細胞の捕捉 (2015)
  • Author(s): 米田和恵、田中文啓
  • Organizer: 第19回日本がん分子標的治療学会学術集会
  • Place of Presentation: 松山全日空ホテル（愛媛県松山市）
  • Year and Date: 2015-06-10
• [Presentation] Capture of EpCAM-negative circulating tumor cells (CTCs) with a “Universal CTC-Chip”. (2015)
  • Author(s): Kazue Yoneda, Yasuhiro Chikaishi, Eri Kawashima, Tomoko So, Hidetaka Uramoto, Takashi Ohnaga, Fumihiro Tanaka.
  • Organizer: AACR Annual Meeting 2015
  • Place of Presentation: Philadelphia（USA)
  • Year and Date: 2015-04-18
  • Int'l Joint Research
• [Presentation] Development of system to detect circulating tumor cell in malignant pleural mesothelioma (2013)
  • Author(s): Yasuhiro Chikaishi, Tomoko So, Masaru Takenaka, Soichi Oka, Makoto Nakagawa, Hidehiko Shimokawa, Teruo Iwatav, Hidetaka Uramoto, Takeshi Hanagiri, Fumihiro Tanaka, Takeshi Ohnaga
  • Organizer: AACR meeting
  • Place of Presentation: Washington,USA
• [Presentation] 循環腫瘍細胞（CTC）の検出方法の開発 (2013)
  • Author(s): 近石泰弘、宗 知子、米田和恵、浦本秀隆、花桐武志、大永 崇、田中文啓
  • Organizer: BioJapan2013
  • Place of Presentation: パシフィコ横浜