Development of a CTC (circulating tumor cell) – guided system for discrimination between multiple lung cancer and pulmonary metastases

• Project/Area Number: 25462192
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory surgery
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: IWATA Teruo 産業医科大学, 医学部, 非常勤医師 (00435124)
• Co-Investigator(Kenkyū-buntansha): YONEDA Kazue 産業医科大学, 医学部, 助教 (80724806) ; URAMOTO Hidetaka 産業医科大学, 医学部, 准教授 (90389445) ; TANAKA Fumihiro 産業医科大学, 医学部, 教授 (10283673)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 肺癌 / 循環腫瘍細胞 / 多発肺癌 / 肺内転移

Outline of Final Research Achievements

Discrimination between multiple lung cancer (MPL) and pulmonary metastases (PM) is clinically important but is sometimes difficult with routine clinic-pathological and imaging modalities. In the current study, we employed circulating tumor cell (CTC) as a marker for the discrimination. We first used “CellSearch” to detect CTCs in patients with multiple lung nodules and with suspicion of MPL or PM, but the CTC-test did not provide any information for the discrimination because CTCs were not detected in most patients. Based on the results, we have tried to develop more sensitive detection system of CTCs, and developed a novel CTC-detection system with microfluidic device (“CTC-chip”). In experimental studies, the “CTC-chip” system provided higher efficiency in capturing tumor cells than “CellSeach”. We now evaluate CTCs in the blood sampled from patients, and will evaluate the diagnostic performance of the CTC-test with the “CTC-chip” in discrimination between MPL and PM.

Research Products

• [Journal Article] Frequency of EGFR mutations in Bangladeshi patients with adenocarcinoma of the lung. (2014)
  • Author(s): Shakib R, Kondo N, Yoneda K, Takuwa T, Hashimoto M, Orui H, Okumura Y, Tanaka F, Kumamoto K, Mostafa M, Chowdhury G, Haque A, Hasegawa S.
  • Journal Title: Int J Clin Oncol Volume: 19 Pages: 45-9
• [Journal Article] A feasibility study of induction pemetrexed plus cisplatin followed by pleurectomy/ decortication aimed at macroscopic complete resection for malignant pleural mesothelioma. (2013)
  • Author(s): Shimokawa M, Hasegawa S, Fukuoka K, Okada M, Yokoi K, Tanaka F, Yamanaka T, Daimon T, Nakano T.
  • Journal Title: Jpn J Clin Oncol Volume: 43 Issue: 5 Pages: 575-8
  • DOI: 10.1093/jjco/hyt035
  • Peer Reviewed
• [Journal Article] Clinical significance of the frequency of regulatory T cells in regional lymph node lymphocytes as a prognostic factor for non-small-cell lung cancer. (2013)
  • Author(s): Hanagiri T1, Shigematsu Y, Shinohara S, Takenaka M, Oka S, Chikaishi Y, Nagata Y, Iwata T, Uramoto H, So T, Tanaka F.
  • Journal Title: Lung Cancer. Volume: 81(3) Issue: 3 Pages: 475-479
  • DOI: 10.1016/j.lungcan.2013.07.001
  • Peer Reviewed
• [Journal Article] Preoperative embolization followed by palliative therapy for patients unable to undergo the complete removal of a chronic expanding hematoma: A report of a case (2013)
  • Author(s): Kuwata T, Uramoto H, Oka S, Hidehiko Shimokawa H, Shigematsu Y, Baba T, Hanagiri T, Tanaka F（8人中2番目）
  • Journal Title: Asian J Surg Volume: 36 Issue: 1 Pages: 40-2
  • DOI: 10.1016/j.asjsur.2012.06.006
  • Peer Reviewed
• [Journal Article] Prognostic implications of human leukocyte antigen class I expression in patients who underwent surgical resection for non-small-cell lung cancer. (2013)
  • Author(s): Hanagiri T, Shigematsu Y, Kuroda K, Baba T, Shiota H, Ichiki Y, Nagata Y, Yasuda M, Uramoto H, So T, Takenoyama M, Tanaka F.
  • Journal Title: J Surg Res Volume: 181(2) Issue: 2 Pages: 57-63
  • DOI: 10.1016/j.jss.2012.07.029
  • Peer Reviewed
• [Journal Article] Intrapleural chemotherapy improves the survival of non-small cell lung cancer patients with positive pleural lavage cytology. (2013)
  • Author(s): Baba T, Uramoto H, Kuwata T, Takenoyama M, Chikaishi Y, Oka S, Nagata Y, Shigematsu Y, Shimokawa H, Nakagawa M, So T, Hanagiri T, Tanaka F.
  • Journal Title: Surg Today Volume: 43 Issue: 6 Pages: 648-53
  • DOI: 10.1007/s00595-012-0281-y
  • NAID: 10031176087
  • Peer Reviewed
• [Journal Article] Serum Level of Osteopontin as a Prognostic Factor in Patients Who Underwent Surgical Resection for Non-Small-Cell Lung Cancer (2013)
  • Author(s): Takenaka M, Hanagiri T, Shinohara S, Yasuda M, Chikaishi Y, Oka S, Shimokawa H, Nagata Y, Nakagawa M, Uramoto H, So T, Yamada S, Tanaka F（13人中10番目）
  • Journal Title: Clin Lung Cancer Volume: 14 Issue: 3 Pages: 288-94
  • DOI: 10.1016/j.cllc.2012.09.005
  • Peer Reviewed
• [Journal Article] Discriminative features of thin-slice CT for peripheral intrapulmonary lymph nodes. (2013)
  • Author(s): Takenaka M, Uramoto H, Shimokawa H, So T, Hanagiri T, Aoki T, Tanaka F.
  • Journal Title: Asian J Surg Volume: 36 Issue: 2 Pages: 69-73
  • DOI: 10.1016/j.asjsur.2012.11.005
  • Peer Reviewed
• [Journal Article] Association of EGFR mutation or ALK rearrangement with expression of DNA repair and synthesis genes in never-smoker women with pulmonary adenocarcinoma. (2013)
  • Author(s): Uramoto H, Tanaka F.
  • Journal Title: Cancer Volume: 119 Issue: 14 Pages: 2660-2660
  • DOI: 10.1002/cncr.28095
  • Peer Reviewed
• [Journal Article] Angiogenesis of lung cancer utilizes existing blood vessels rather than developing new vessels using signals from carcinogenesis (2013)
  • Author(s): Uramoto H, Yamada S, Tanaka F
  • Journal Title: Anticancer Res Volume: 33 Pages: 1913-6
  • Peer Reviewed
• [Journal Article] Cancer/testis antigen expression as a predictor for epidermal growth factor receptor mutation and prognosis in lung adenocarcinoma (2012)
  • Author(s): Baba T, Tanaka F(8人中8番目)
  • Journal Title: Eur J Cardiothorac Surg. Volume: 43 Issue: 4 Pages: 759-764
  • DOI: 10.1093/ejcts/ezs426
  • Peer Reviewed
• [Presentation] Improved efficacy in capturing EpCAM-negative tumor cells using a “Universal CTC-chip” (2015)
  • Author(s): Kazue Yoneda, Yasuhiro Chikaishi, Eri Kawashima, Takashi Ohnaga, Fumihiro Tanaka.
  • Organizer: AACR Tumor metastasis 2015
  • Place of Presentation: Austin, USA
  • Year and Date: 2015-11-30
  • Int'l Joint Research
• [Presentation] Capture of EpCAM-negative circulating tumor cells (CTCs) with a “Universal CTC-Chip” (2015)
  • Author(s): Kazue Yoneda, Yasuhiro Chikaishi, Eri Kawashima, Tomoko So, Hidetaka Uramoto, Takashi Ohnaga, Fumihiro Tanaka.
  • Organizer: AACR Annual Meeting 2015
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2015-04-18
  • Int'l Joint Research
• [Presentation] 左膿胸及び左荒蕪肺に対し一期的に左下葉切除と広背筋による膿胸腔充填を施行した1例 (2013)
  • Author(s): 岩田輝男, 松本崇秀, 名部祐介, 門司祥子, 平良彰浩, 竹中 賢、 近石泰弘, 岡 壮一, 永田好香, 下川秀彦, 中川 誠, 浦本秀隆, 宗 知子, 花桐武志, 田中 文啓
  • Organizer: 第30回日本呼吸器外科学会総会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 放射線治療後の超高齢心不全腎不全合併肺癌に肺動脈形成術施行し切除し得た1例 (2013)
  • Author(s): 岩田輝男, 松本崇秀, 名部祐介, 門司祥子, 平良彰浩, 竹中 賢、 近石泰弘, 岡 壮一, 永田好香, 下川秀彦, 中川 誠, 浦本秀隆, 宗 知子, 花桐武志, 田中 文啓
  • Organizer: 第30回日本呼吸器外科学会総会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 右上葉肺癌気管支浸潤と炎症性気道狭窄に対して右上中葉管状切除術を施行した1例 (2013)
  • Author(s): 岩田輝男, 松本崇秀, 名部祐介, 門司祥子, 平良彰浩, 竹中 賢、 近石泰弘, 岡 壮一, 永田好香, 下川秀彦, 中川 誠, 浦本秀隆, 宗 知子, 花桐武志, 田中 文啓
  • Organizer: 第30回日本呼吸器外科学会総会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 難治性気管狭窄に対し気管管状切除術を施行した1例 (2013)
  • Author(s): 岩田輝男, 竹中 賢, 近石泰弘, 岡 壮一平井文子, 岩浪崇嗣, 永田好香,下川秀彦 宗 知子, 浦本秀隆, 花桐武志, 田中文啓
  • Organizer: 第3回福岡胸部外科疾患研究会
  • Place of Presentation: 博多
• [Presentation] 呼吸器外科周術期管理について (2013)
  • Author(s): 岩田輝男
  • Organizer: 院内講演
  • Place of Presentation: 小倉記念病院
• [Presentation] VATS縦隔リンパ節生検における器具の工夫 (2013)
  • Author(s): 岩田輝男, 藤本拓也, 福山啓太, 野田智宏, 下池典広, 多田誠一郎, 前川久継, 吉本裕紀, 藤川高久, 安部俊弘, 田中 明
  • Organizer: 4th 北九州 Chest surgical forum
  • Place of Presentation: TKP小倉シティセンター
• [Presentation] 肺癌 (2013)
  • Author(s): 岩田輝男
  • Organizer: 院内がん専門看護師講演
  • Place of Presentation: 小倉記念病院
• [Presentation] 左残肺全摘中のPA根部からの出血 (2013)
  • Author(s): 岩田輝男, 名部祐介, 門司祥子, 平良彰浩, 竹中 賢, 近石泰弘, 岡 壮一, 平井文子, 岩浪崇嗣, 永田好香, 下川秀彦, 宗 知子, 浦本秀隆, 田中文啓
  • Organizer: 第3回Graham Peaarson Cooper Patterson conference
  • Place of Presentation: ホテル白菊、別府
• [Book] 肺癌 (2013)
  • Author(s): 岩田輝男, 桑田泰治, 岩浪崇嗣, 浦本秀隆, 田中文啓
  • Publisher: 日本肺癌学会
• [Book] 日本呼吸器外科学会雑誌 (2013)
  • Author(s): 門司祥子, 岩田輝男, 宗 知子, 浦本秀隆, 花桐武志, 田中文啓
  • Publisher: 日本呼吸器外科学会