Mechanisms of analgesic action by granulocyte colony-stimulating factor in the spinal dorsal horn

• Project/Area Number: 25462303
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Wakayama Medical University
• Principal Investigator: Tsutsui Shunji 和歌山県立医科大学, 医学部, 講師 (70423960)
• Co-Investigator(Kenkyū-buntansha): Taniguchi Wataru 和歌山県立医科大学, 医学部, 助教 (20453194)
• Co-Investigator(Renkei-kenkyūsha): Sonekatsu Mayumi 和歌山県立医科大学, 医学部, 学内助教 (40725579) ; Nishio Naoko 和歌山県立医科大学, 医学部, 特別研究員 (40648359)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 顆粒球コロニー刺激因子 / G-CSF / パッチクランプ法 / in vivoパッチクランプ法 / 脊髄後角 / シグナル伝達 / 興奮性シナプス後電流 / 抑制性シナプス後電流 / パッチクランプ

Outline of Final Research Achievements

We investigated effects of Granulocyte colony-stimulating factor (G-CSF) onto the synaptic transmission of the rat spinal dorsal horn neurons by patch-clamp methods. In our study, bath application of G-CSF tended to enhance spontaneous excitatory postsynaptic currents (sEPSCs) of dorsal horn neurons. On the other hand, G-CSF had no effect onto spontaneous inhibitory postsynaptic currents (sIPSCs) of dorsal horn neurons. Moreover, we analyzed how G-CSF affected dorsal horn neurons by in vivo patch-clamp. It showed that sEPSCs were slightly inhibited by bath application of G-CSF. These results suggest that dorsal horn neurons have cell-specific response to G-CSF. We think that inhibitory effects onto excitatory neurotransmission by G-CSF lead analgesic actions.

Research Products

• [Journal Article] Interferon-gamma potentiates NMDA receptor signaling in spinal dorsal horn neurons via microglia-neuron interaction. (2016)
  • Author(s): Sonekatsu M, Taniguchi W, Yamanaka M, Nishio N, Tsutsui S, Yamada H, Yoshida M, Nakatsuka T.
  • Journal Title: Molecular Pain Volume: 12 Pages: 1-12
  • DOI: 10.1177/1744806916644927
  • Peer Reviewed / Open Access
• [Journal Article] Can decompression surgery relieve low back pain in patients with lumbar spinal stenosis combined with degenerative lumbar scoliosis? (2013)
  • Author(s): Tsutsui S, Kagotani R, Yamada H, Hashizume H, Minamide A, Nakagawa Y, Iwasaki H, Yoshida M
  • Journal Title: Eur Spine J. Volume: 22 Issue: 9 Pages: 2010-2014
  • DOI: 10.1007/s00586-013-2786-5
  • Peer Reviewed
• [Journal Article] Quantification of the proportion of motor neurons recruited by transcranial electrical stimulation during intraoperative motor evoked potential monitoring. (2013)
  • Author(s): Tsutsui S, Yamada H, Hashizume H, Minamide A, Nakagawa Y, Iwasaki H, Yoshida M
  • Journal Title: J Clin Monit Comput. Volume: 27 Issue: 6 Pages: 633-637
  • DOI: 10.1007/s10877-013-9480-3
  • Peer Reviewed
• [Journal Article] P2X 受容体を介した慢性疼痛メカニズムと鎮痛 (2013)
  • Author(s): 谷口亘, 吉田宗人, 中塚映政.
  • Journal Title: 臨床整形外科 Volume: 48 Pages: 1169-1174
  • Peer Reviewed
• [Journal Article] Reactive oxygen species enhance excitatory synaptic transmission in rat spinal dorsal horn neurons by activating TRPA1 and TRPV1 channels. (2013)
  • Author(s): Nishio N, Taniguchi W, Sugimura YK, Takiguchi N, Yamanaka M, Kiyoyuki Y, Yamada H, Miyazaki N, Yoshida M, Nakatsuka T
  • Journal Title: Neuroscience Volume: 247 Pages: 201-12
  • DOI: 10.1016/j.neuroscience.2013.05.023
  • Peer Reviewed
• [Presentation] The role of NMDA receptor activation by IFNγ in the spinal dorsal horn neurons (2016)
  • Author(s): Sonekatsu M, Taniguchi W, Yamanaka M, Nishio N, Tsutsui S, Nishi H, Hashizume H, Yamada H, Yoshida M, Nakatsuka T.
  • Organizer: Orthopedic Research Society 2016 Annual meeting
  • Place of Presentation: Orlando, Florida（アメリカ）
  • Year and Date: 2016-03-01
  • Int'l Joint Research
• [Presentation] Interferon-gamma activates NMDA receptors in the dorsal horn of spinal cord (2015)
  • Author(s): Sonekatsu M, Taniguchi W, Yamanaka M, Nishio N, Tsutsui S, Hashizume H, Yoshida M, Nakatsuka T.
  • Organizer: Society for Neuroscience Annual meeting 2015
  • Place of Presentation: Chicago（アメリカ）
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] 1. Feasibility of novel transcranial electrical stimulation technique in intraoperative neurophysiologic monitoring during spinal surgery: Multi-train stimulation. (2013)
  • Author(s): Tsutsui S, Iwasaki H, Yamada H, Hashizume H, Minamide A, Nakagawa Y, Nishi H, Yoshida M
  • Organizer: Cevical spine research society
  • Place of Presentation: Los Angeles, USA
• [Book] 最新医学 71(2) 痛みのClinical Neuroscience 8脊髄機能変化と痛み：アロディニアなどのメカニズムを巡って. (2016)
  • Author(s): 谷口亘、中塚映政
  • Total Pages: 4
  • Publisher: 最新医学社
• [Book] 先端医療シリーズ44 臨床医のための最新整形外科 (2013)
  • Author(s): 谷口亘, 中塚映政.
  • Total Pages: 4
  • Publisher: 先端医療技術研究所
• [Book] 痛みのScience ＆Practice シリーズ２「痛みの薬物治療」 (2013)
  • Author(s): 谷口亘, 中塚映政
  • Total Pages: 9
  • Publisher: 文光堂
• [Book] Neuroprotection and Regeneration of the Spinal Cord (2013)
  • Author(s): Wataru Taniguchi, Terumasa Nakatsuka.
  • Total Pages: 12
  • Publisher: Springer Japan