Effect of parathyroid hormone on chondrogenic differentiation from mesenchymal stem cells

• Project/Area Number: 25462347
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Yokohama City University
• Principal Investigator: KUMAGAI Ken 横浜市立大学, 医学部, 講師 (10468176)
• Co-Investigator(Kenkyū-buntansha): SAITO Tomoyuki 横浜市立大学, 医学研究科, 教授 (30170517)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 軟骨分化 / PTH / 間葉系幹細胞 / MSC / 副甲状腺ホルモン

Outline of Final Research Achievements

The present study demonstrated that chondrogenic differentiation of Mesenchymal stem cells (MSCs) was modulated by parathyroid hormone (PTH). The results revealed that PTH has opposite effects on chondrogenesis when administered at different concentrations. Namely, low to moderate concentrations of PTH promoted chondrogenic differentiation of MSCs, whereas chondrogenesis of MSCs was inhibited and not stimulated by a higher concentration of PTH. Based on clinical experience with the efficacy and safety of PTH for bone metabolism, PTH may also be useful clinically for cartilage repair.