NF-kB inhibitory molecule mediated TNF responsiveness of rheumatoid arthritis delivered fibroblast-like synoviocyte
| Project/Area Number |
25462390
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 関節リウマチ / 滑膜線維芽細胞 / NF-kappaB / ABIN-3 / NEDD4L / ユビキチンリガーゼ / アポトーシス / ABIN / ユビキチン / E3ユビキチンリガーゼ / TNFR2 / TNFα / DNAチップ |
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| Outline of Final Research Achievements |
To elucidate the molecular mechanisms for the pro-inflammatory effect of ABIN-3, an inhibitor for NF-κB activation, we established the stable transfectant constitutively expressing ABIN-3 by enforced expression in fibroblast-like synoviocyte cell line MH7A derived from rheumatoid arthritis patient designated as A3G. A3G showed the phenotypic changes as spindle-like appearance, growth retardation, decreased expression of MMPs and ALDH1A1, and down-regulated activity of Erk and Akt. We identified E3 ubiquitin ligase NEDD4L as a binding partner of ABIN-3 by mass-spectrometry and enforced expression.
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Report
(3 results)
Research Products
(5 results)
