The molecular mechanism of effects of midazolam on dendritic cells and immune response
| Project/Area Number |
25462434
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology
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| Research Institution | Osaka University |
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Principal Investigator |
Ohta Noriyuki 大阪大学, 医学部附属病院, 助教 (60379162)
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| Co-Investigator(Kenkyū-buntansha) |
FUJINO Yuji 大阪大学, 大学院医学系研究科(研究院), 教授 (50252672)
後藤 幸子 大阪大学, 医学(系)研究科(研究院), 助教 (20276710)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 樹状細胞 / Translocator protein / 免疫 / マクロファージ / TSPO / ベンゾジアゼピン / IL-6 / 一酸化窒素 / ミダゾラム / テトラマー / トランスジェニックマウス |
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| Outline of Final Research Achievements |
Benzodiazepine shows sedative effect that suppresses central nerve system via GABA receptor. On the other hand benzodiazepine has another functional molecule as translocator protein (TSPO). Benzodiazepine was reported that affects function of immune cells, but it is not uncertain about those of functional mechanism. We report that we found the mechanism of midazolam on immune cells. Using a murine macrophage cell line, RAW264 cells, we analyzed the effect of midazolam on the expression of CD80 and CD86 and the secretion of IL-6. Midazolam suppressed the expression of costimulatory molecules and IL-6. The activation of RAW264 was inhibited by TSPO ligand but not by GABA legends. Finally we analyzed the contribution of TSPO by analysis of using knockdown RAW264 cells by siRNA for TSPO. The effect of midazolam was inhibited by knockdown of TSPO. These results showed that midazolam has an inhibitory action of immune response on murine macrophages via TSPO.
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Report
(3 results)
Research Products
(4 results)
