Chromatin remodeling and reprogramming with oocyte specific linker histone,

• Project/Area Number: 25462580
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Keio University (2015); St. Marianna University School of Medicine (2013)
• Principal Investigator: Tanaka Mamoru 慶應義塾大学, 医学部, 教授 (20207145)
• Co-Investigator(Kenkyū-buntansha): 河村 和弘 聖マリアンナ医科大学, 医学部, 准教授 (10344756)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: リンカーヒストン / 卵子 / 初期化 / H1foo

Outline of Final Research Achievements

Oocyte-specific linker histone H1foo is expressed in oocyte and early stage embryos. H1foo is essential for in vitro meiotic maturation of oocyte. The greater mobility of H1foo, compared with H1, may contribute to this rapid replacement and the instability of chromatin structure. These findings suggest that the rapid replacement of H1 with H1foo may play an important role in nuclear remodeling.