Diabetic retinopathy treatment targeting PPAR gamma

• Project/Area Number: 25462717
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: The University of Tokushima
• Principal Investigator: MITAMURA Yoshinori 徳島大学, 大学院医歯薬学研究部, 教授 (30287536)
• Co-Investigator(Kenkyū-buntansha): HOTTA Fumika 徳島大学, 病院, 医員 (80645838) ; EGAWA Mariko 徳島大学, 大学院医歯薬学研究部, 講師 (70507657) ; MINO Akiko 徳島大学, 病院, 助教 (50531836)
• Co-Investigator(Renkei-kenkyūsha): HARADA Takayuki 公益財団法人東京都医学総合研究所, 視覚病態プロジェクト, 副参事研究員 (90345306)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 外科 / 細胞・組織 / 生体分子 / 糖尿病 / 臨床

Outline of Final Research Achievements

Peroxisome proliferator-activated receptor gamma (PPARγ) was detected in the exosome fraction of the culture media of human umbilical vein endothelial cells, indicating that PPARγ, a nuclear protein, can be released into the extracellular fluid. PPARγ mRNA expression levels in membrane samples, and the PPARγprotein concentrations in aqueous humor and vitreous fluid samples were significantly higher in patients with proliferative diabetic retinopathy (PDR) than control patients. We observed a significant positive correlation between the PPARγlevels and vascular endothelial growth factor levels or clinical stage of retinopathy in the aqueous humor and vitreous fluid of PDR patients. Taken together, PPARγ may be a therapeutic target for diabetic retinopathy.

Research Products

• [Journal Article] Correlation between optic nerve head circulation and visual function before and after anti-VEGF therapy for central retinal vein occlusion: prospective, interventional case series. (2016)
  • Author(s): Nagasato D, Mitamura Y, Semba K, Akaiwa K, Nagasawa T, Yoshizumi Y, Tabuchi H, Kiuchi Y
  • Journal Title: BMC Ophthalmol Volume: 16 Issue: 1 Pages: 36-36
  • DOI: 10.1186/s12886-016-0211-7
  • NAID: 120006814103
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Changes of choroidal structure after treatment for primary intraocular lymphoma: retrospective, observational case series (2015)
  • Author(s): Egawa M, Mitamura Y, Sano H, Akaiwa K, Niki M, Semba K, Sonoda S, Sakamoto T
  • Journal Title: BMC Ophthalmol Volume: 15 Issue: 1 Pages: 136-136
  • DOI: 10.1186/s12886-015-0127-7
  • Peer Reviewed / Open Access
• [Journal Article] Relationship between retinal microstructures and visual acuity after cataract surgery in patients with retinitis pigmentosa. (2015)
  • Author(s): Nakamura Y, Mitamura Y, Hagiwara A, Kumagai K, Miura G, Sugawara T, Egawa M, Yamamoto S
  • Journal Title: Br J Ophthalmol Volume: 99 Issue: 4 Pages: 508-511
  • DOI: 10.1136/bjophthalmol-2013-304819
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Binarization of enhanced depth imaging optical coherence tomographic images of an eye with Wyburn-Mason syndrome: a case report. (2015)
  • Author(s): Iwata A, Mitamura Y, Niki M, Semba K, Egawa M, Katome T, Sonoda S, Sakamoto T
  • Journal Title: BMC Ophthalmol Volume: 15 Issue: 1 Pages: 19-19
  • DOI: 10.1186/s12886-015-0014-2
  • NAID: 120006862763
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Expression of intraocular peroxisome proliferator-activated receptor gamma in patients with proliferative diabetic retinopathy. (2015)
  • Author(s): Katome T, Namekata K, Mitamura Y, Semba K, Egawa M, Naito T, Harada C, Harada T
  • Journal Title: J Diabetes Complications Volume: 29 Issue: 2 Pages: 275-281
  • DOI: 10.1016/j.jdiacomp.2014.10.010
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Case of adult-onset Coats' disease with epiretinal membrane treated with 25-gauge pars plana vitrectomy (2015)
  • Author(s): Mino A, Mitamura Y, Katome T, Semba K, Egawa M, Naito T
  • Journal Title: The Journal of Medical Investigation Volume: 62 Issue: 1.2 Pages: 85-88
  • DOI: 10.2152/jmi.62.85
  • NAID: 130005129702
  • ISSN: 1343-1420, 1349-6867
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Renin-angiotensin system regulates neurodegeneration in a mouse model of normal tension glaucoma (2015)
  • Author(s): Semba, K., Namekata, K., Guo, X., Harada, C., Harada, T. and Mitamura, Y
  • Journal Title: Cell Death and Disease Volume: 5 Issue: 7 Pages: e1333-e1333
  • DOI: 10.1038/cddis.2014.296
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prepapillary vascular loops complicated by suspected macroaneurysm rupture. (2014)
  • Author(s): Akaiwa K, Mitamura Y, Katome T, Semba K, Egawa M, Naito T
  • Journal Title: Case Rep Ophthalmol Med Volume: 2014 Pages: 157242-157242
  • DOI: 10.1155/2014/157242
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dock3 overexpression and p38 MAPK inhibition synergistically stimulate neuroprotection and axon regeneration after optic nerve injury. (2014)
  • Author(s): Semba K, Namekata K, Kimura A, Harada C, Katome T, Yoshida H, Mitamura Y, Harada, T
  • Journal Title: Neurosci Lett Volume: 581 Pages: 89-93
  • DOI: 10.1016/j.neulet.2014.08.034
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Brimonidine prevents neurodegeneration in a mouse model of normal tension glaucoma. (2014)
  • Author(s): Semba K, Namekata K, Kimura A, Harada C, Mitamura Y, Harada T
  • Journal Title: Cell Death Dis Volume: 5 Issue: 7 Pages: e1341-e1341
  • DOI: 10.1038/cddis.2014.306
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Changes of fundus autofluorescence and spectral-domain optical coherence tomographic findings after treatment of primary intraocular lymphoma. (2014)
  • Author(s): Egawa M, Mitamura Y, Hayashi Y, Semba K, Naito T
  • Journal Title: J Ophthalmic Inflamm Infect Volume: 4 Issue: 1 Pages: 7-7
  • DOI: 10.1186/1869-5760-4-7
  • Peer Reviewed