Exploratory research about intraocular inflammation refractory marker using newly developed carbohydrate structural analysis; Glycoblottoing

• Project/Area Number: 25462745
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Hokkaido University
• Principal Investigator: NAMBA KENICHI 北海道大学, 大学病院, 講師 (70333599)
• Co-Investigator(Kenkyū-buntansha): KITAICHI NOBUYOSHI 北海道医療大学, 個体差医療科学センター, 教授 (40431366) ; NISHIMURA SHINICHIRO 北海道大学, 先端科学研究科(研究院), 教授 (00183898) ; AMANO MAHO 北海道大学, 先端生命科学研究科(研究院), 特任助教 (80365808)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 網膜ぶどう膜炎 / ベーチェット病 / 糖鎖解析 / 炎症増悪マーカー

Outline of Final Research Achievements

In this study we examined about the specific carbohydrate chains associated with refractory intraocular inflammations. Glycoblottoing which was newly developed carbohydrate structural analysis was performed with the serum of 2 groups; 1) Behcet’s disease patients who were refractory to infliximab treatment and were positive of anti-infliximab antibody. 2) Behcet’s disease patients who were well controlled with infliximab treatment. Four carbohydrate chains; m/z1680, m/z2337, m/z2379, m/z3195 were detected with significant difference between 2 groups, and all of them were increased in refractory patients.

Research Products

• [Journal Article] A case of tuberculous endophthalmitis successfully treated with vitrectomy followed by antituberculous agents (2015)
  • Author(s): Hase K, Namba K, Saito W, Ohno S, Ishida S
  • Journal Title: J Ophthalmic Inflamm Infect Volume: 5 Issue: 1 Pages: 14-14
  • DOI: 10.1186/s12348-015-0046-z
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Significant role of the choroidal outer layer during recovery from choroidal thickening in Vogt-Koyanagi-Harada disease patients treated with systemic corticosteroids (2015)
  • Author(s): Hirooka K, Saito W, Namba K, Mizuuchi K, Iwata D, Hashimoto Y, Ishida S
  • Journal Title: 10.1186/s12886-015-0171-3 Volume: 15 Issue: 1 Pages: 181-181
  • DOI: 10.1186/s12886-015-0171-3
  • NAID: 120005702356
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Relationship between choroidal blood flow velocity and choroidal thickness during systemic corticosteroid therapy for Vogt-Koyanagi-Harada disease (2015)
  • Author(s): Hirooka K, Saito W, Namba K, Takemoto Y, Mizuuchi K, Uno T, Tagawa Y, Hashimoto Y, Ishida S
  • Journal Title: Graefes Arch Clin Exp Ophthalmol Volume: 253 Issue: 4 Pages: 609-617
  • DOI: 10.1007/s00417-014-2927-5
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Single center study on ethnic and clinical features of Behcet's disease in Moscow, Russia. (2015)
  • Author(s): Lennikov A, Alekberova Z, Goloeva R, Kitaichi N, Denisov L, Namba K, Takeno M, Ishigatsubo Y, Mizuki N, Nasonov E, Ishida S, Ohno S.
  • Journal Title: Clin Rheumatol Volume: 34 Issue: 2 Pages: 321-327
  • DOI: 10.1007/s10067-013-2442-9
  • Peer Reviewed
• [Journal Article] Acute zonal occult outer retinopathy in Japanese patients: Clinical features, visual function, and factors affecting visual function (2015)
  • Author(s): Saito S, Saito W, Saito M, Hashimoto Y, Mori S, Noda K, Namba K, Ishida S
  • Journal Title: PLoS One Volume: 10 Issue: 4 Pages: e0125133-e0125133
  • DOI: 10.1371/journal.pone.0125133
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Development and validation of new diagnostic criteria for acute retinal necrosis. (2015)
  • Author(s): Takase H, Okada AA, Goto H, Mizuki N, Namba K, Ohguro N, Sonoda KH, Tomita M, Keino H, Kezuka T, Kubono R, Mizuuchi K, Shibuya E, Takahashi H, Yanai R, Mochizuki M
  • Journal Title: Jpn J Ophthalmol Volume: 59(1) Issue: 1 Pages: 14-20
  • DOI: 10.1007/s10384-014-0362-0
  • NAID: 210000165197
  • Peer Reviewed / Open Access
• [Journal Article] A Major Review: Current Aspects of Ocular Behçet's Disease in Japan. (2015)
  • Author(s): Namba K, Goto H, Kaburaki T, Kitaichi N, Mizuki N, Asukata Y, Fujino Y, Meguro A, Sakamoto S, Shibuya E, Yokoi K, Ohno S.
  • Journal Title: Ocul Immunol Inflamm. Volume: 23 Suppl 1 Issue: sup1 Pages: S1-S23
  • DOI: 10.3109/09273948.2014.981547
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A case of mature natural killer-cell neoplasm manifesting multiple choroidal lesions -Primary intraocular NK- cell lymphoma (2015)
  • Author(s): Tagawa Y, Namba K, Ogasawara R, Kanno H, Ishida S
  • Journal Title: Case Rep Ophthalmol Volume: 21 Issue: 3 Pages: 380-384
  • DOI: 10.1159/000442018
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Amelioration of experimental autoimmune uveoretinitis by inhibition of glyceraldehyde derived-advanced glycation end product formation. (2014)
  • Author(s): Dong Z, Iwata D, Kitaichi N, Takeuchi M, Sato M, Endo N, Iwabuchi K, Ando R, Fukuhara J, Kinoshita S, Lennikov A, Kitamura M, Mizuuchi K, Kanda A, Noda K, Namba K, Yamagishi S, Ohno S, Ishida S
  • Journal Title: J Leukoc Biol Volume: 96 Issue: 6 Pages: 1077-1085
  • DOI: 10.1189/jlb.3a0513-288rrr
  • Peer Reviewed / Open Access
• [Journal Article] The Iternational Criteria for Behçet's Disease (ICBD): a collaborative study of 27 countries on the sensitivity and specificity of the new criteria (2014)
  • Author(s): International Team for the Revision of the International Criteria for Behcet's Disease (ITR-ICBD), Davatchi F, Assaad-Khalil S, Calamia KT, Crook JE, Sadeghi-Abdollahi B, Namba K（42番目）et al 計63名
  • Journal Title: J Eur Acad Dermatol Venereol Volume: 28 Issue: 3 Pages: 338-347
  • DOI: 10.1111/jdv.12107
  • Peer Reviewed / Open Access
• [Journal Article] Behçet's disease ocular attack score 24: evaluation of ocular disease activity before and after initiation of infliximab. (2014)
  • Author(s): Kaburaki T, Namba K, Sonoda KH, Kezuka T, Keino H, Fukuhara T, Kamoi K, Nakai K, Mizuki N, Ohguro N; Ocular Behçet Disease Research Group of Japan
  • Journal Title: Jpn J Ophthalmol Volume: 58(2) Issue: 2 Pages: 120-130
  • DOI: 10.1007/s10384-013-0294-0
  • NAID: 40020019721
  • Peer Reviewed / Open Access
• [Journal Article] Amelioration of endotoxin-induced uveitis treated with the sea urchin pigment echinochrome in rats. (2014)
  • Author(s): Lennikov A, Kitaichi N, Noda K, Mizuuchi K, Ando R, Dong Z, Fukuhara J, Kinoshita S, Namba K, Ohno S, Ishida S
  • Journal Title: Mol Vis Volume: 20 Pages: 171-177
  • NAID: 120005427742
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of the long-term efficacy and safety of infliximab treatment for uveitis in Behçet's disease: a multicenter study. (2014)
  • Author(s): Takeuchi M, Kezuka T, Sugita S, Keino H, Namba K, Kaburaki T, Maruyama K, Nakai K, Hijioka K, Shibuya E, Komae K, Hori J, Ohguro N, Sonoda KH, Mizuki N, Okada AA, Ishibashi T, Goto H, Mochizuki M
  • Journal Title: Ophthalmology Volume: 121(10) Issue: 10 Pages: 1877-1884
  • DOI: 10.1016/j.ophtha.2014.04.042
  • Peer Reviewed / Open Access
• [Journal Article] Acute retinal necrosis: factors associated with anatomic and visual outcomes. (2013)
  • Author(s): Mizuki N
  • Journal Title: Jpn J Ophthalmol Volume: 57 Issue: 1 Pages: 98-103
  • DOI: 10.1007/s10384-012-0211-y
  • NAID: 10031168363
  • Peer Reviewed
• [Journal Article] Bacterial endophthalmitis caused by an intraocular cilium in a patient under treatment with infliximab. (2013)
  • Author(s): Jin XH, Namba K, Saito W, Iwata D, Ishida S.
  • Journal Title: J Ophthalmic Inflamm Infect Volume: 3 Issue: 1 Pages: 50-50
  • DOI: 10.1186/1869-5760-3-50
  • Peer Reviewed
• [Journal Article] Clinical features of human T lymphotropic virus type-1 associated uveitis in Hokkaido, Japan (2013)
  • Author(s): Kase S, Namba K, Kitaichi N, Iwata D, Ohno S, Ishida S
  • Journal Title: Jpn J Ophthalmo Volume: Vol.57 Issue: 4 Pages: 379-384
  • DOI: 10.1007/s10384-013-0244-x
  • NAID: 10031189427
  • Peer Reviewed
• [Journal Article] Genome-wide association study identifies GIMAP as a novel susceptibility locus for Behcet's disease. (2013)
  • Author(s): Lee YJ, Horie Y, Wallace GR, Choi YS, Park JA, Song R, Kang YM, Kang SW, Baek HJ, Kitaichi N, Meguro A, Mizuki N, Namba K, Ishida S, Kim J, Niemczek E, Lee EY, Song YW, Ohno S, Lee EB
  • Journal Title: Ann Rheum Dis Volume: 72 Pages: 1510-15016
  • Peer Reviewed
• [Journal Article] Induction of heat shock protein 70 ameliorates ultraviolet-induced photokeratitis in mice. (2013)
  • Author(s): Lennikov A, Kitaichi N, Kase S, Noda K, Horie Y, Nakai A, Ohno S, Ishida S
  • Journal Title: Int J Mol Sci Volume: 14 Issue: 1 Pages: 2175-2189
  • DOI: 10.3390/ijms14012175
  • NAID: 120005228266
  • Peer Reviewed
• [Journal Article] Trabecular meshwork depigmentation in Vogt-Koyanagi-Harada disease. (2013)
  • Author(s): Mizuuchi K, Kitaichi N, Namba K, Horie Y, Ishida S, Ohno S
  • Journal Title: Jpn J Ophthalmol Volume: 57 Issue: 3 Pages: 245-251
  • DOI: 10.1007/s10384-012-0229-1
  • NAID: 10031176278
  • Peer Reviewed
• [Journal Article] Two cases of subfoveal choroidal neovascularization with tubulointerstitial nephritis and uveitis (TINU) syndrome. (2013)
  • Author(s): Takemoto Y, Namba K, Mizuuchi K, Ohno S, Ishida S.
  • Journal Title: Eur J Ophthalmol Volume: 23 Issue: 2 Pages: 255-257
  • DOI: 10.5301/ejo.5000240
  • Peer Reviewed
• [Presentation] Clinicopathological study on epiretinal membrane of ocular sarcoidosis (2015)
  • Author(s): Kase S, Namba K, Mizuuchi K, Iwata D, Kitaichi N, Ohno S, Ishida S
  • Organizer: AAO (American Academy of Ophthalmology)2015
  • Place of Presentation: Sands Expo (Las Vegas, USA)
  • Year and Date: 2015-11-14
  • Int'l Joint Research
• [Presentation] Diagnostic efficacy of cell block method for vitreoretinal lymphoma (2015)
  • Author(s): Kase S, Namba K, Iwata D, Mizuuchi K, Ishida S.
  • Organizer: 13th IOIS (International Ocular Inflammation Society)
  • Place of Presentation: Hilton San Francisco Union Square (San Francisco, USA)
  • Year and Date: 2015-09-25
  • Int'l Joint Research
• [Presentation] Choroidal circulation. Symposium: Imaging in Behcet’s disease (2015)
  • Author(s): Namba K
  • Organizer: Uveitis Workshop 2015 / Asia-Pacific Intraocular Inflammation Study Group Meeting
  • Place of Presentation: Radission Blu Hotel Cebu (Cebu, Philippines)
  • Year and Date: 2015-05-15
  • Int'l Joint Research
• [Presentation] The appearance of antibodies to infliximab in the course of infliximab administration in Behcet’s disease (2014)
  • Author(s): Namba K
  • Organizer: 9th Japan-Korea International Symposium in Ophthalmology
  • Place of Presentation: Hokkaido University（Sapporo, Japan）
  • Year and Date: 2014-12-01
• [Presentation] 抗インフリキシマブ抗体価の上昇とともに眼発作と投与時反応がみられたベーチェット病 (2014)
  • Author(s): 水内一臣、南場研一、竹本裕子、田川義晃、宇野友絵、岩田大樹、北市伸義、大野重昭、石田 晋
  • Organizer: 第68回日本日本臨床眼科学会
  • Place of Presentation: 神戸ポートピアホテル（神戸市）
  • Year and Date: 2014-11-13 – 2014-11-16
• [Presentation] HLA-B*51 carrier is susceptible to Behcet’s disease and its ocular lesions in East Asian population (2014)
  • Author(s): Horie Y, Meguro A, Ohta T, Lee EB, Namba K, Mizuki N, Ishida S, Ohno S, Kitaichi N.
  • Organizer: 16th International Conference on Behcet’s disease
  • Place of Presentation: Cite Internationale Universitaire de Paris（Paris, France）
  • Year and Date: 2014-09-18 – 2014-09-20
• [Presentation] Amelioration of endotoxin-induced uveitis treated with the sea urchin pigment echinochrome (2014)
  • Author(s): Kitaichi N, Lennikov A, Noda K, Mizuuchi K, Namba K, Ohno S, Ishida S
  • Organizer: 16th International Conference on Behcet’s disease
  • Place of Presentation: Cite Internationale Universitaire de Paris（Paris, France）
  • Year and Date: 2014-09-18 – 2014-09-20
• [Presentation] The appearance of antibodies to infliximab in the course of infliximab administration in Behcet’s disease (2014)
  • Author(s): Namba K, Mizuuchi K, Takemoto Y, Tagawa Y, Uno T, Fukuhara T, Ohno S, Kitaichi N, Ishida S
  • Organizer: 16th International Conference on Behcet’s disease
  • Place of Presentation: Cite Internationale Universitaire de Paris（Paris, France）
  • Year and Date: 2014-09-18 – 2014-09-20
• [Presentation] Ocular Behcet Disease Research Group of Japan．新しいBehcet病ぶどう膜炎の活動性スコアによる評価の再現性の検討 (2014)
  • Author(s): 蕪城 俊克、南場 研一、園田 康平、毛塚 剛司、慶野 博、福原 崇子、鴨居 功樹、中井 慶
  • Organizer: 第118回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム（Tokyo, Japan）
  • Year and Date: 2014-04-02 – 2014-04-06
• [Presentation] Reasons of the application of infliximab therapy to the patients with ocular behcet’s disease (2014)
  • Author(s): Kaburaki T, Namba K, Sonoda K, Kezuka T, Keino H.
  • Organizer: World Ophtalmology Congress 2014 Tokyo
  • Place of Presentation: 東京国際フォーラム（Tokyo, Japan）
  • Year and Date: 2014-04-02 – 2014-04-04
• [Presentation] Correlation between ocular inflammatory attacks and serum trough level of infliximab or serum concentration of cytokines in the clinical course of behcet’s disease patients with infliximab treatment (2014)
  • Author(s): Mizuuchi K, Namba K, Takemoto Y, Ohno S, Ishida S
  • Organizer: World Ophtalmology Congress 2014 Tokyo
  • Place of Presentation: 東京国際フォーラム（Tokyo, Japan）
  • Year and Date: 2014-04-02 – 2014-04-04
• [Presentation] Recent advances in the etiopathogenesis and management of ocular Behcet's disease : Novel scoring system of ocular lesions in Behcet's disease (2014)
  • Author(s): Namba K.
  • Organizer: World Ophtalmology Congress 2014 Tokyo
  • Place of Presentation: 東京国際フォーラム（Tokyo, Japan）
  • Year and Date: 2014-04-02 – 2014-04-04
  • Invited
• [Presentation] A Case of tuberculous endophthalmitis. (2013)
  • Author(s): Hase K, Namba K, Saito W, Takemoto Y, Mizuuchi K, Ohno S, Ishida S.
  • Organizer: International Workshop on Granulomatous Uveitis and Ocular Behcet's disease and Asia-Pacific Intraocular Inflammation Study Group
  • Place of Presentation: Han-Hsien International Hotel（Kaohsiung, Taiwan）
• [Presentation] ぶどう膜炎を合併した急性帯状潜在性網膜外層症の1例 (2013)
  • Author(s): 宇野友絵、南場研一、齋藤 航、水内一臣、福原崇子、田川義晃、石田 晋
  • Organizer: 第67回日本臨床眼科学会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] ベーチェット病眼発作スコア24によるインフリキシマブ治療前後の疾患活動性の評価 (2013)
  • Author(s): 蕪城俊克、南場研一、園田康平、毛塚剛司、慶野 博、福原崇子、鴨居功樹、中井 慶、水木信久、大黒伸行
  • Organizer: 第67回日本臨床眼科学会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] 平滑筋肉腫による腫瘍随伴網膜症の1例 (2013)
  • Author(s): 高橋明裕、齋藤 航、岩田大樹、齋藤理幸、南場研一、神田敦宏、石田 晋
  • Organizer: 第67回日本臨床眼科学会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] ぶどう膜炎に続発した血管増殖性網膜腫瘍の2症例 (2013)
  • Author(s): 水内一臣、南場研一、齋藤 航、堀江幸弘、田川義晃、宇野友絵、竹本裕子、福原崇子、北市伸義、石田 晋
  • Organizer: 第67回日本臨床眼科学会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] 両眼に多発性脈絡膜腫瘤を呈したNK細胞リンパ腫の一例 (2013)
  • Author(s): 1. 田川 義晃、南場 研一、水内 一臣、宇野 友絵、加瀬 諭、福原 崇子 齋藤 航 、石田 晋
  • Organizer: 第31回日本眼腫瘍学会
  • Place of Presentation: 総合あんしんセンター（高知市）
• [Presentation] 血管新生緑内障を合併した眼内悪性リンパ腫の2例 (2013)
  • Author(s): 福原淳一、加瀬 諭、野田実香、南場研一、畑中佳奈子、石田 晋
  • Organizer: 第31回日本眼腫瘍学会
  • Place of Presentation: 総合あんしんセンター（高知市）
• [Presentation] 原発性眼内悪性リンパ腫に対する初回拡大治療の効果 (2013)
  • Author(s): 福原崇子、吉川 洋、武田篤信、吉村 武、吉本五一、宮本敏浩、南場研一、石田 晋、石橋竜朗
  • Organizer: 第31回日本眼腫瘍学会
  • Place of Presentation: 総合あんしんセンター（高知市）
• [Presentation] 脈絡膜新生血管を合併しベバシズマブ硝子体注射を要した小児原田病の1例 (2013)
  • Author(s): 田川義晃、南場研一、齋藤 航、水内一臣、北市伸義、大野重昭、石田 晋
  • Organizer: フォーサム 2013 大阪
  • Place of Presentation: 大阪国際会議場（大阪市）
• [Presentation] 周辺虹彩前癒着を伴うぶどう膜炎に続発した緑内障に対するsuture-trabeculotomyの成績 (2013)
  • Author(s): 大口剛司、新明康弘、新田卓也、山本麻梨亜、南場研一、大野重昭、陳 進輝、石田 晋
  • Organizer: 第117回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム（東京都千代田区）
• [Presentation] ぶどう膜炎における網羅的糖鎖解析 (2013)
  • Author(s): 南場研一、北市伸義、安藤 亮、竹本裕子、水内一臣、堀江幸弘、大野重昭、天野麻穂、西村紳一郎、石田 晋.
  • Organizer: 第117回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム（東京都千代田区）
• [Presentation] ベーチェット病眼炎症発作とインフリキシマブトラフ値・サイトカイン血中濃度との相関 (2013)
  • Author(s): 水内一臣、竹本裕子、南場研一、宇野友絵、大野重昭、石田 晋
  • Organizer: 第117回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム（東京都千代田区）