Basic approach to elucidate pathogenesis and develop innovative therapy for anti-aquapolin-4 seropositive optic neuritis.
| Project/Area Number |
25462760
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
松永 芳径 東京医科大学, 医学部, 兼任助教 (20421050)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 抗アクアポリン4抗体陽性視神経炎 / 視神経脊髄炎 / 二次元電気泳動 / アストロサイト / マウス実験的自己免疫性視神経炎 / タンパク解析 / 樹状細胞療法 / GFAP |
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| Outline of Final Research Achievements |
A human CGRP-expressing plasmid was constructed, and used to transfect mouse bone marrow-derived matured DC (mDC) by electroporation methods. For gene therapy, Experimental autoimmune optic neuritis (EAON) developed in 50% of mice in the CGRP-transfected group compared with 80% in the mock-transfected group, and the mean pathological score for EAON was 1 in the CGRP transfected group compared with 2 in the mock-transfected group. IL-10 production by spleen cells in the CGRP-transfected group increased independent of MOG concentration, compared with the mock-transfected group. Collected serum from the patients with anti-aquapolin-4 seropositive optic neuritis was measured the protein level using 2D-DIGE. The protein levels of AMBP and Nicolin were elevated in the serum collected from the patients with anti-aquapolin-4 seropositive optic neuritis.
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Report
(5 results)
Research Products
(24 results)
