The role of PKR on the differentiation of osteoblasts and formation of osteoclasts
| Project/Area Number |
25462859
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
HANEJI Tatsuji 徳島大学, 大学院医歯薬学研究部, 教授 (50156379)
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| Co-Investigator(Kenkyū-buntansha) |
MORIMOTO Hiroyuki 産業医科大学, 医学部, 教授 (30335806)
TERAMACHI Jyumpei 徳島大学, 大学院医歯薬研究部, 助教 (20515986)
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| Co-Investigator(Renkei-kenkyūsha) |
OKAMURA Hirohiko 徳島大学, 大学院医歯薬研究部, 准教授 (20380024)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 骨芽細胞 / 破骨細胞 / 骨形成 / 骨吸収 / 人工的歯周炎 / PKR / LPS / 骨代謝 / TNF-α / NF-κB / ＰＫＲ / 炎症性骨疾患 |
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| Outline of Final Research Achievements |
We investigated the roles of PKR in osteoclast formation and bone resorption in vitro and in vivo. LPS induced high levels of PKR in osteoclast precursor cells. The LPS-induced osteoclast differentiation was markedly suppressed in the cells pre-treated with PKR inhibitor, C16 and in the cells in which gene silencing of PKR were done. Inhibition of PKR activity in the cells was suppressed the expression of osteoclast differentiation markers including c-fos and NFATc. Bone resorption activity of the LPS-induced osteoclasts was also suppressed by the C16 treatment. Inhibition of PKR activity suppressed the LPS-induced activation of NF-κB and MAPK in osteoclasts. Treatment of C16 effectively prevented the LPS-induced destruction of alveolar bone in the artificial periodontitis in rat. Thus, the present results suggest that PKR plays a pivotal role in the LPS-induced bone loss in periodontitis and PKR inhibition may become an important therapeutic target for periodontitis.
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Report
(4 results)
Research Products
(58 results)
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[Journal Article] Measles virus nucleocapsid protein increases osteoblast differentiation in Paget's disease2016
Author(s)
Teramachi J, Nagata Y, Mohammad K, Inagaki Y, Ohata Y, Guise T, Michou L, Brown JP, Windle JJ, Kurihara N, Roodman GD
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Journal Title
Journal of Clinical Investigation
Volume: 126
Issue: 3
Pages: 1012-1022
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Blocking the ZZ domain of sequestosome1/p62 suppresses myeloma growth and osteoclast formation in vitro and induces dramatic bone formation in myeloma-bearing bones in vivo2016
Author(s)
Teramachi J, Silbermann R, Yang P, Zhao W, Mohammad KS, Guo J, Anderson JL, Zhou D, Feng R, Myint KZ, Maertz N, Beumer JH, Eiseman JL, Windle JJ, Xie XQ, Roodman GD, Kurihara N
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Journal Title
Leukemia
Volume: 30
Issue: 2
Pages: 390-398
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Effective impairment of myeloma cells and their progenitors by blockade of monocarboxylate transportation2015
Author(s)
6. Hanson DJ, Nakamura S, Amachi R, Hiasa M, Oda A, Tsuji D, Itoh K, Harada T, Horikawa K, Teramachi J, Miki H, Matsumoto T, Abe M.
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Journal Title
Oncotarget
Volume: 6
Issue: 32
Pages: 33568-33586
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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