| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We investigated the functional role of Ca2+-activated K+ channels (Kca) in osteoclastic bone resorption. Mature osteoclasts derived from mouse bone marrow cells abundantly expressed Kca 3.1. Pharmacological inhibition of Kca 3.1 led to rapid disappearance of ring-like actin conformation (actin-ring) and reduced bone resorption activity. Other Kca blockers had no effect on both the actin-ring conformation and the bone resorption activity. Mature osteoclasts also expressed non-selective cation channels including TRPM7. Inhibition of these cation channels also reduced the bone resorption activity. These results suggest that Kca 3.1 expressed in mature osteoclasts serves as a regulator of Ca2+ influx through the non-selective cation channels, which contribute to the formation of actin-ring, an essential process for the bone resorption.
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