The role of the Mediator complexes in transcriptional regulation of the chemokine genes

Research Project

Project/Area Number	25462928
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Pathobiological dentistry/Dental radiology
Research Institution	Meikai University
Principal Investigator	Ohmori Yoshihiro 明海大学, 歯学部, 教授 (50194311)
Co-Investigator(Kenkyū-buntansha)	Hiroi Miki 明海大学, 歯学部, 講師 (30419717)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	転写制御 / ケモカイン / インターフェロン / メディエーター複合体 / CDK8 / STAT1 / CXCL9 / CXCL10 / NF-kB / Mediator / NF-κB
Outline of Final Research Achievements	Inflammatory reactions and immune responses are regulated by a diverse array of extracellular stimuli such as cytokines in the tissue microenvironment. The purpose of this study is to elucidate the mechanisms how the extracellular stimuli induce the inflammatory genes including chemokines. Inflammatory cytokines TNFα and IFNγ synergistically induced the expression of chemokine CXCL9, CXCL10 genes and the Mediator complex, which functions to integrate transcriptional activators and RNA polymerase II, negatively regulated the chemokine gene expressions.