| Project/Area Number |
25463229
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Periodontology
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Yohei 日本大学, 松戸歯学部, 専任講師 (30434088)
TAKAI Hideki 日本大学, 松戸歯学部, 専任講師 (30453898)
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| Research Collaborator |
MATSUMURA Hiroyoshi
MATSUI Sari
KATO Ayako
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | microRNA / サイトカイン / 遺伝子発現 / タンパク質発現 / 歯肉線維芽細胞 / 炎症 / 歯周炎 / miRNA / 歯周病 / 炎症性サイトカイン / IL-1 / IL-6 / MKP-5 / IKKα / miR-223 / 歯肉 / 結合組織 / 上皮 |
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| Outline of Final Research Achievements |
MicroRNAs (miRNAs) can bind to target mRNAs through partial complementarity and regulate many genes. To determine the effects of miR-223 on the expressions of IL-1β and IL-6, HGF were stimulated by IL-1β (1 ng/mL) or TNFα (10 ng/mL) and transfected with a miR-223 expression plasmid. Levels of mRNA for IL-1β, IL-6, inhibitor of kappa-B kinase α (IKKα) and mitogen-activated protein kinase phosphatase-5 (MKP-5) were measured by real-time PCR, and levels IL-1β, IL-6 and IKKα protein were determined by enzyme-linked immunosorbent assay and Western blotting. Expression of IL-1β and IL-6 mRNAs was induced by IL-1β and TNFα and further increased by miR-223 overexpression. IL-1β and TNFα induced the expression of IL-1β and IL-6 mRNAs, and this was reduced by miR-223 inhibitor. Overexpression of miR-223 decreased the levels of IKKα protein and MKP-5 mRNA in HGF. These findings indicate that miR-223 might control the inflammatory response via IKKα and MKP-5 in periodontal tissue.
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