Role of RNA molecules in the polycomb complex, PRC1
Project/Area Number |
25503004
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
epigenetics
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Research Institution | Institute of Physical and Chemical Research |
Principal Investigator |
Masui Osamu 国立研究開発法人理化学研究所, 統合生命医科学研究センター, 研究員 (30579305)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | ポリコーム複合体 / RNA / Xist / エピジェネティクス |
Outline of Final Research Achievements |
In this study, we tried to address how Polycomb repressive complex 1 (PRC1) suppresses its target genes via RNA molecule(s). We performed the RNA immuno-precipitation (RIP) assay using the antibody against Ring1B protein that is a catalytic subunit of PRC1, and discovered (1) PRC1 (Ring1B) binds to Xist RNA and mRNAs that code components of PRC1 itelf, and (2) the binding between PRC1 and these RNAs is not mediated by Ring1B or Cbx proteins but an unknown 120 kDa protein.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] A Pooled shRNA Screen Identifies Rbm15, Spen, and Wtap as Factors Required for Xist RNA-Mediated Silencing2015
Author(s)
Moindrot B, Cerase A, Coker H, Masui O, Grijzenhout A, Pintacuda G, Schermelleh L, Nesterova TB, Brockdorff N
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Journal Title
Cell Reports
Volume: 12
Issue: 4
Pages: 562-572
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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