Development of infrared laser atmospheric pressure ionization techniques for high throughput mass spectrometry of membrane proteins
| Project/Area Number |
25513004
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
オミクス計測科学
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| Research Institution | Osaka University |
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Principal Investigator |
HAZAMA Hisanao 大阪大学, 工学(系)研究科(研究院), 准教授 (70437375)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | レーザーイオン化質量分析 / 赤外線レーザー / 大気圧イオン化 / 液体クロマトグラフィー / 膜タンパク質 |
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| Outline of Final Research Achievements |
Atmospheric pressure ion source for online coupling of liquid chromatography (LC) and mass spectrometry (MS) was developed using an infrared laser and a porous metal thin disk called frit. Peptides dissolved in water or mixture of water and acetonitrile were successfully ionized and analyzed with MS by tuning the laser wavelength to the absorption peak of the solvent. Moreover, online LC/MS of a mixture of peptides was succeeded.
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Report
(4 results)
Research Products
(21 results)
