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Development of Efficient Expansion for Human Pluripotent Stem Cells

Research Project

Project/Area Number 25560195
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionKyoto University

Principal Investigator

KAWASE Eihachiro  京都大学, 再生医科学研究所, 講師 (70402790)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords幹細胞 / ES細胞 / iPS細胞 / 大量培養 / ヒト多能性幹細胞 / ヒトES細胞 / ヒトiPS細胞 / 大量培養法 / 再生医療 / 多能性幹細胞 / 未分化維持 / 化合物ライブラリ
Outline of Final Research Achievements

Human pluripotent stem cells (hPSCs) including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) is thought to provide renewable source for a wide range of applications in regenerative medicine, and as tools for human disease modeling and drug discovery. For these purposes, large numbers of cells with high quality are essential. Recently, we show that biological substrate, recombinant E8 fragments of laminin isoforms (LM-E8s) sustain long-term self-renewal of hPSCs in defined, zeno-freee media with dissociated single-cell passing. However, biological materials are generally expensive to manufacture, have limited scalability and may have batch-to-batch variability. In this study, we developed our culture system to show similar performance using LM-E8s to efficient expansion of hPSCs under defined, xeno-free conditions using non-biological synthetic substrates.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (15 results)

All 2015 2014 2013

All Journal Article (6 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 3 results,  Open Access: 3 results) Presentation (7 results) (of which Invited: 1 results) Book (2 results)

  • [Journal Article] Efficient and scalable culture of single dissociated human pluripotent stem cells using recombinant E8 fragments of human laminin isoforms.2015

    • Author(s)
      Miyazaki, T. and Kawase, E
    • Journal Title

      Curr. Protoc. Stem Cell Biol.

      Volume: 32:1C. 18 Pages: 1-8

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Selective elimination of human pluripotent stem cells by a marine natural product derivative.2014

    • Author(s)
      Ting-Fang Kuo, Di Mao, Nao Hirata, Bilon Khambu, Yasuhisa Kimura, Eihachiro Kawase, Hiroki Shimogawa, Makoto Ojika, Norio Nakatsuji, Kazumitsu Ueda, and Motonari Uesugi
    • Journal Title

      J Am Chem Soc

      Volume: 136 Issue: 28 Pages: 9798-9801

    • DOI

      10.1021/ja501795c

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Efficient Expansion of Dissociated Human Pluripotent Stem Cells using a Synthetic Substrate2014

    • Author(s)
      Kawase, E.
    • Journal Title

      Methods Mol. Biol.

      Volume: - Pages: 61-69

    • DOI

      10.1007/7651_2014_82

    • ISBN
      9781493926671, 9781493926688
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A chemical probe that labels human pluripotent stem cells2014

    • Author(s)
      Hirata N, Nakagawa M, Fujibayashi Y, Yamauchi K, Murata A, Minami I, Tomioka M, Kondo T, Kuo TF, Endo H, Inoue H, Sato S, Ando S, Kawazoe Y, Aiba K, Nagata K, Kawase E, Chang YT, Suemori H, Eto K, Nakauchi H, Yamanaka S, Nakatsuji N, Ueda K, Uesugi M
    • Journal Title

      Cell reports

      Volume: 6 Issue: 6 Pages: 1165-74

    • DOI

      10.1016/j.celrep.2014.02.006

    • NAID

      120005466668

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of small molecules that promote human embryonic stem cell self-renewal.2013

    • Author(s)
      Kumagai, H., Suemori, H., Uesugi, M., Nakatsuji, N. and Kawase, E
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 434 Issue: 4 Pages: 710-716

    • DOI

      10.1016/j.bbrc.2013.03.061

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Multidisciplinary Research of Human Pluripotent Stem Cells for Application to Cell Therapy and Drug Discovery2013

    • Author(s)
      Norio Nakatsuji, Eihachiro Kawase, Takamichi Miyazaki, Itsunari Minami and Kazuhiro Aiba
    • Journal Title

      Tissue Engineering and Regenerative Medicine

      Volume: 10 Issue: 4 Pages: 160-163

    • DOI

      10.1007/s13770-013-0019-y

    • Related Report
      2013 Research-status Report
  • [Presentation] A NEW METHOD FOR THE DETECTION OF RESIDUAL MOUSE FEEDER CELLS CONTAMINATED IN THE PRODUCTS OF HUMAN PLURIPOTENT STEM CELLS.2014

    • Author(s)
      Takahashi, Tsuneo A., Tanaka, Keiji, Okuda, Shinji, Hirai, Masako, Takada, Kei, Kawase, Eihachiro, Suemori, Hirofumi, Nakatsuji, Norio, Kitagawa, Masanari.
    • Organizer
      12th International Society for Stem Cell Research
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      2014-06-18 – 2014-06-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] HIGH FREQUENCY OF INTRACELLULAR ICE FORMATION DURING SLOW COOLING IN COLONIES OF HUMAN EMBRYONIC STEM CELLS AND INDUCED PLURIPOTENT STEM CELLS: COMPARISON TO DISSOCIATED SINGLE CELLS.2014

    • Author(s)
      Takada, Kei, Hirai, Masako, Hamao, Mari, Kashigi, Fumi, Kawase, Eihachiro, Surmori Hirofumi, Nakatsuji, Norio. Takahashi, Tsuneo.
    • Organizer
      12th International Society for Stem Cell Research
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      2014-06-18 – 2014-06-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] VALIDATION OF CLINICAL GRADE HUMAN EMBRYONIC STEM CELLS PRODUCED UNDER GMP.2014

    • Author(s)
      Hirai, Masako, Takada, Kei, Hamao, Mari, Kashigi, Fumi, Kawase, Eihachiro, Surmori Hirofumi, Nakatsuji, Norio. Takahashi, Tsuneo.
    • Organizer
      12th International Society for Stem Cell Research
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      2014-06-18 – 2014-06-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒトES/iPS細胞調製における残存マウスフィーダー細胞の測定法の開発2014

    • Author(s)
      高橋恒夫、田中啓二、奥田真治、平井雅子、高田圭、川瀬栄八郎、末盛博文、中辻憲夫、北川 正成
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒトES細胞既存株のクリーンアップによる臨床用ヒトES細胞株バンキングの構築2014

    • Author(s)
      平井雅子、高田圭、濱生麻里、樫木芙美、川瀬栄八郎、末盛博文、中辻憲夫、高橋恒夫
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒトES細胞における凍結保存の解析:コロニーの高頻度の細胞内氷晶形成2014

    • Author(s)
      高田圭、平井雅子、川瀬栄八郎、末盛博文、中辻憲夫、高橋恒夫
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] pplications of scalable culture system toward for clinical-graded undifferentiated human embryonic stem cells.2013

    • Author(s)
      Kawase E.
    • Organizer
      UST-Kyoto iCeMS International Symposium
    • Place of Presentation
      National Chiao Tung University
    • Related Report
      2013 Research-status Report
    • Invited
  • [Book] “Development of Culture Substrates and Culture Media for Efficient Expansion of Human Pluripotent Stem Cells.” In Lecture Notes on Cell Biology and Materials Sciences”2014

    • Author(s)
      Kawase, E.
    • Total Pages
      9
    • Publisher
      University System of Taiwan
    • Related Report
      2014 Annual Research Report
  • [Book] 「ES・iPS細胞のフィーダーフリー培養法」実験医学 別冊「ES・iPS細胞実験スタンダード」より2014

    • Author(s)
      宮崎隆道、川瀬栄八郎
    • Total Pages
      9
    • Publisher
      羊土社
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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