Development of Efficient Expansion for Human Pluripotent Stem Cells
Project/Area Number |
25560195
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Biomedical engineering/Biomaterial science and engineering
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Research Institution | Kyoto University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | 幹細胞 / ES細胞 / iPS細胞 / 大量培養 / ヒト多能性幹細胞 / ヒトES細胞 / ヒトiPS細胞 / 大量培養法 / 再生医療 / 多能性幹細胞 / 未分化維持 / 化合物ライブラリ |
Outline of Final Research Achievements |
Human pluripotent stem cells (hPSCs) including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) is thought to provide renewable source for a wide range of applications in regenerative medicine, and as tools for human disease modeling and drug discovery. For these purposes, large numbers of cells with high quality are essential. Recently, we show that biological substrate, recombinant E8 fragments of laminin isoforms (LM-E8s) sustain long-term self-renewal of hPSCs in defined, zeno-freee media with dissociated single-cell passing. However, biological materials are generally expensive to manufacture, have limited scalability and may have batch-to-batch variability. In this study, we developed our culture system to show similar performance using LM-E8s to efficient expansion of hPSCs under defined, xeno-free conditions using non-biological synthetic substrates.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Selective elimination of human pluripotent stem cells by a marine natural product derivative.2014
Author(s)
Ting-Fang Kuo, Di Mao, Nao Hirata, Bilon Khambu, Yasuhisa Kimura, Eihachiro Kawase, Hiroki Shimogawa, Makoto Ojika, Norio Nakatsuji, Kazumitsu Ueda, and Motonari Uesugi
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Journal Title
J Am Chem Soc
Volume: 136
Issue: 28
Pages: 9798-9801
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A chemical probe that labels human pluripotent stem cells2014
Author(s)
Hirata N, Nakagawa M, Fujibayashi Y, Yamauchi K, Murata A, Minami I, Tomioka M, Kondo T, Kuo TF, Endo H, Inoue H, Sato S, Ando S, Kawazoe Y, Aiba K, Nagata K, Kawase E, Chang YT, Suemori H, Eto K, Nakauchi H, Yamanaka S, Nakatsuji N, Ueda K, Uesugi M
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Journal Title
Cell reports
Volume: 6
Issue: 6
Pages: 1165-74
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] A NEW METHOD FOR THE DETECTION OF RESIDUAL MOUSE FEEDER CELLS CONTAMINATED IN THE PRODUCTS OF HUMAN PLURIPOTENT STEM CELLS.2014
Author(s)
Takahashi, Tsuneo A., Tanaka, Keiji, Okuda, Shinji, Hirai, Masako, Takada, Kei, Kawase, Eihachiro, Suemori, Hirofumi, Nakatsuji, Norio, Kitagawa, Masanari.
Organizer
12th International Society for Stem Cell Research
Place of Presentation
Vancouver, Canada
Year and Date
2014-06-18 – 2014-06-21
Related Report
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[Presentation] HIGH FREQUENCY OF INTRACELLULAR ICE FORMATION DURING SLOW COOLING IN COLONIES OF HUMAN EMBRYONIC STEM CELLS AND INDUCED PLURIPOTENT STEM CELLS: COMPARISON TO DISSOCIATED SINGLE CELLS.2014
Author(s)
Takada, Kei, Hirai, Masako, Hamao, Mari, Kashigi, Fumi, Kawase, Eihachiro, Surmori Hirofumi, Nakatsuji, Norio. Takahashi, Tsuneo.
Organizer
12th International Society for Stem Cell Research
Place of Presentation
Vancouver, Canada
Year and Date
2014-06-18 – 2014-06-21
Related Report
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[Presentation] VALIDATION OF CLINICAL GRADE HUMAN EMBRYONIC STEM CELLS PRODUCED UNDER GMP.2014
Author(s)
Hirai, Masako, Takada, Kei, Hamao, Mari, Kashigi, Fumi, Kawase, Eihachiro, Surmori Hirofumi, Nakatsuji, Norio. Takahashi, Tsuneo.
Organizer
12th International Society for Stem Cell Research
Place of Presentation
Vancouver, Canada
Year and Date
2014-06-18 – 2014-06-21
Related Report
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