Chemomechanical network modeling for molecular motor dynamics of kinesin
Project/Area Number |
25610121
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Biological physics/Chemical physics/Soft matter physics
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Research Institution | Okayama University |
Principal Investigator |
Sumi Tomonari 岡山大学, 自然科学研究科, 准教授 (40345955)
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Research Collaborator |
Klumpp Stefan Max-Planck Institute (MPI) of Colloids and Interfaces
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | 分子モーター / 化学-力学共役機構 / 確率的モデリング / 力学的破断過程 / 一分子観測 / キネシン / 溶媒和自由エネルギー / 密度汎関数理論 / 単一分子計測 / ダイニン |
Outline of Final Research Achievements |
We present a chemomechanical network model of molecular motor kinesin that is systematically developed on the basis of experimental observations, and demonstrate that it can qualitatively reproduce available all the experimental results of motor dynamics. The analysis of the local excess fluxes shows that an ATP-concentration dependence of the dominant forward stepping cycle and (2) supports a gated rear head mechanism where the forward step is controlled by ATP hydrolysis and the resulting ADP-bound state of the rear head at saturating ATP levels. The network picture suggests that for saturating ATP concentration, the energy from ATP hydrolysis is used to concentrate the chemical transition flux on the network to a force-generating state that can produce the power stroke. At low ATP levels, the energy is used to avoid states that are fragile against backward load because the leading head is weakly bound to microtubule.
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Report
(4 results)
Research Products
(35 results)
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[Presentation] 界面における疎水効果2015
Author(s)
甲賀研一郎,阿部紀遥,墨智成
Organizer
第66回コロイドおよび界面化学討論会
Place of Presentation
鹿児島大学
Year and Date
2015-09-10
Related Report
Invited
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[Presentation] 蛋白質間相互作用と溶媒効果2013
Author(s)
墨智成, 今村比呂志, 森田剛, 磯貝泰弘, 西川恵子
Organizer
2013年日本化学会中国四国支部:若手セッション企画「機能性ソフトマテリアルと分子統計化学」
Place of Presentation
広島大学
Related Report
Invited
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