Study on deep biosphere ecosystem in oceanic crust by "single-cell" chemical speciation

• Project/Area Number: 25610164
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Geochemistry/Cosmochemistry
• Research Institution: Ehime University (2015); University of Shizuoka (2013-2014)
• Principal Investigator: Mitsunobu Satoshi 愛媛大学, 農学部, 准教授 (70537951)
• Co-Investigator(Kenkyū-buntansha): SHIRAISHI Fumito 広島大学, 大学院理学研究科, 助教 (30626908)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: シングルセル / スペシエーション / 微生物 / 金属 / シングルセル分析 / 鉄酸化バクテリア / 玄武岩 / パイライト / X線顕微鏡

Outline of Final Research Achievements

It is much difficult to investigate a deep biosphere microbial ecosystem in oceanic crust due to their low activities and difficulty in the analysis. Hence, there is little finding even on general microbial metabolic process of the deep biosphere. In this study, we have developed a new method to analyze chemical species of metals and organic materials at single-cell level by coupling two advanced techniques, scanning transmission X-ray microscopy and fluorescence in situ hybridization.

Research Products

• [Journal Article] Direct Detection of Fe(II) in Extracellular Polymeric Substances (EPS) at the Mineral-Microbe Interface in Bacterial Pyrite Leaching (2016)
  • Author(s): Mitsunobu S., Zhu M., Takeichi Y., Ohigashi T., Suga H., Jinno M., Makita H., Sakata M., Ono K., Mase K., Takahashi Y.
  • Journal Title: Microbes and Environments Volume: 31 Issue: 1 Pages: 63-69
  • DOI: 10.1264/jsme2.ME15137
  • NAID: 130005138797
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Nano-scale identification of extracellular organic substances at microbe-mineral interface by scanning transmission X-ray microscopy (STXM) (2015)
  • Author(s): Mitsunobu S, Zhu M, Takeichi Y, Ohigashi T, Suga H, Makita H, Sakata M, Ono K, Mase K, Takahashi Y
  • Journal Title: Chemistry Letters Volume: 44 Pages: 91-93
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Presence of oxygen and aerobic communities from sea floor to basement in deep-sea sediments (2015)
  • Author(s): D’Hondt, S., Inagaki, F., Zarikian, C.A., Yamaguchi, Y.T., et al.
  • Journal Title: Nature Geoscience Volume: 8 Issue: 4 Pages: 299-304
  • DOI: 10.1038/ngeo2387
  • Peer Reviewed
• [Journal Article] Arsenic attenuation in geothermal streamwater coupled with biogenic arsenic(III) oxidation (2013)
  • Author(s): Mitsunobu S., Hamamura N., Kataoka T., Shiraishi F.
  • Journal Title: Applied Geochemistry Volume: 35 Pages: 154-160
  • NAID: 130004594046
  • Peer Reviewed
• [Journal Article] Behavior of antimony(V) during the transformation of ferrihydrite and its environmental implications (2013)
  • Author(s): Mitsunobu S., Muramatsu C., Watanabe K., Sakata M.
  • Journal Title: Environmental Science & Technology Volume: 47 Pages: 9660-9667
  • Peer Reviewed
• [Journal Article] Effectiveness of sulfur and boron isotopes in aerosols as tracers of emissions from coal burning in Asian continent. (2013)
  • Author(s): Sataka M., Ishikawa T., Mitsunobu S.
  • Journal Title: Atmospheric Environment Volume: 67 Pages: 296-303
  • Peer Reviewed
• [Journal Article] A new method to observe in situ microbe-metal-mineral interaction by micro-XAFS-FISH technique (2013)
  • Author(s): Mitsunobu S., Shiraishi F.
  • Journal Title: SPring-8 Research Frontiers Volume: 2013 Pages: 102-103
• [Presentation] Nanoscale identification of organic substances at microbe-mineral interface in bacterial leaching of pyrite by STXM (2015)
  • Author(s): Mitsunobu, S., Zhu, M., Takeichi, Y., Ohigashi, T, Suga, H., Sakata, M., Ono, K., Takahashi, Y.
  • Organizer: International Conference on the Biogeochemistry of Trace Elements 2015
  • Place of Presentation: (Fukuoka convention center, Fukuoka
  • Year and Date: 2015-07-12
  • Int'l Joint Research
• [Presentation] 走査型透過X線顕微鏡の微生物－金属－鉱物相互作用解明への応用 (2014)
  • Author(s): 光延聖, 朱鳴, 坂田昌弘, 高橋嘉夫, 武市泰男, 小野寛太
  • Organizer: 2014年度日本地球化学会年会
  • Place of Presentation: 富山大学
  • Year and Date: 2014-09-16 – 2014-09-18
• [Presentation] 走査型透過X線顕微鏡(STXM) の微生物－鉱物相互作用解明への応用 (2014)
  • Author(s): 光延聖, 朱鳴, 高橋嘉夫, 武市泰男, 小野寛太
  • Organizer: 2014年度日本地球惑星科学連合大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-04-28 – 2014-05-02
• [Presentation] マイクロXAFS-FISH法による微生物－金属－鉱物相互作用の直接観察 (2013)
  • Author(s): 光延 聖，白石史人
  • Organizer: 日本地球惑星科学連合2013年会
  • Place of Presentation: 千葉幕張メッセ
  • Invited
• [Presentation] Coupled m-XAFS-FISH technique for direct observation of the microbe-metal-mineral interaction (2013)
  • Author(s): Mitsunobu S, Shiraishi F.
  • Organizer: Goldschmidt2013
  • Place of Presentation: フィレンツェ国際会議場
• [Presentation] X線吸収分光法を駆使したアンチモンやヒ素などの地球表層での挙動に関する研究 (2013)
  • Author(s): 光延 聖
  • Organizer: 日本地球化学会2013年会
  • Place of Presentation: 筑波大学
  • Invited
• [Presentation] 微生物のヒ素酸化反応によって促進される堆積物へのヒ素固定―スペシエーションと機能遺伝子解析による新たなアプローチ (2013)
  • Author(s): 光延 聖，濱村奈津子，片岡剛文，白石史人，坂田昌弘
  • Organizer: 日本地球化学会2013年会
  • Place of Presentation: 筑波大学