Quantification of methanogenic potential with coenzyme F430 analysis
Project/Area Number |
25610166
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Geochemistry/Cosmochemistry
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Research Institution | National Institute of Advanced Industrial Science and Technology (2015) Japan Agency for Marine-Earth Science and Technology (2013-2014) |
Principal Investigator |
Kaneko Masanori 国立研究開発法人産業技術総合研究所, 地圏資源環境研究部門, 主任研究員 (80633239)
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Research Collaborator |
OHKOUCH Naohiko 海洋研究開発機構, 生物地球化学分野, 分野長
TAKANO Yoshinori 海洋研究開発機構, 生物地球化学分野, 主任研究員
NARAOKA Hiroshi 九州大学, 理学研究院, 教授
SHIMA Seigo Max Planck Institutes, Microbial Protein Structure, Research Group Leader
WATANABE Kenji 名古屋大学, 農学部, 講師
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | 補酵素F430 / 機能特異分子分析 / メタン生成 / 嫌気的メタン酸化 / 液体クロマトグラフィートリプル四重極質量分析 / メタン生成アーキア / メタン生成バイオマス / メタン生成-アーキア / 海洋堆積物 / メタン / LC-MS/MS分析 |
Outline of Final Research Achievements |
During the research period, analytical method was developed to quantify coenzyme F430 from various environmental samples. The sensitivity of the developed method is 100000 times higher than the conventional method, which can quantify coenzyme F430 at 0.1 femto mol. The mothod made it possible to quantify coenzyme F430 from marine sediments, microbial mats, paddy soils, groundwater etc. Coenzyme F430 was detected coalbeds located at 2000 m below seafloor offshore Shimokita Peninsula (world record of deep biosphere), suggested presence of active methanogens. This study was published from Science. The scientific results was published from totally 4 peer reviewed scientific journals and presented at 11 conferences and received high international evaluation.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Estimation of methanogenesis by quantification of coenzyme F430 in marine sediments.2016
Author(s)
Kaneko, M., Takano, Y., Ogawa, N.O., Sato, Y., Yoshida, Y., Ohkouchi, N.
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Journal Title
Geochemical Journal
Volume: 50
Pages: in press
DOI
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Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Quantitative Analysis of Coenzyme F430 in Environmental Samples: A New Diagnostic Tool for Methanogenesis and Anaerobic Methane Oxidation2014
Author(s)
Masanori Kaneko, Yoshinori Takano, Yoshito Chikaraishi, Nanako O. Ogawa, Susumu Asakawa, Takeshi Watanabe, Seigo Shima, Martin Kruger, Makoto Matsushita, Hiroyuki Kimura, Naohiko Ohkouchi
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Journal Title
Analytical Chemistry
Volume: 86
Pages: 3633-3638
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] メタン生成場と規模を測る2013
Author(s)
金子雅紀・高野淑識・小川奈々子・大河内直彦
Organizer
日本有機地球化学シンポジウム
Place of Presentation
倉敷市芸文館、岡山県
Year and Date
20130819-20130821
Related Report
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