Imaging analysis of neural circuit involved in voluntary behaviors in C. elegans
Project/Area Number |
25640004
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Neurophysiology / General neuroscience
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Research Institution | Saitama University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
NAKAI Junichi 埼玉大学, 理工学研究科, 教授 (80237198)
OHKURA Masamichi 埼玉大学, 理工学研究科, 准教授 (70369172)
HASHIMOTO Kouichi 東北大学, 情報科学研究科, 教授 (80228410)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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Keywords | イメージング / G-CaMP / 線虫 / オプトジェネティクス |
Outline of Final Research Achievements |
Most Animals can initiate and regulate voluntary behaviors in the absence of external stimuli, but the mechanism underlying decision-making is largely unknown. To elucidate the molecular and neural mechanism of these behaviors, we focused on C. elegans, which have a simple brain consisting of only 302 neurons and transparent body. Using our recently developed G-CaMP6-8, green genetically encoded calcium indicators (GECIs), we developed a system that allowed systematic imaging and analysis of the neural activity during spontaneous locomotion. We further designed R-CaMP2, a high-affinity, fast, red GECI, and successfully achieved dual-color imaging and optical stimulation in combination with green GECIs and channelrhodopshin 2 in freely moving C. elegans.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Rational design of a high-affinity, fast, red calcium indicator R-CaMP2.2015
Author(s)
Inoue M, Takeuchi A, Horigane S, Ohkura M, Gengyo-Ando K, Fujii H, Kamijo S, Takemoto-Kimura S, Kano M, Nakai J, Kitamura K, Bito H
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Journal Title
Nat Methods
Volume: 12
Issue: 1
Pages: 64-70
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C. elegans glycogene database.2014
Author(s)
Akiyoshi S, Nomura KH, Dejima K, Murata D, Matsuda A, Kanaki N, Takaki T, Mihara H, Nagaishi T, Furukawa S, Ando KG, Yoshina S, Mitani S, Togayachi A, Suzuki Y, Shikanai T, Narimatsu H, Nomura K.
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Journal Title
Glycobiology
Volume: 25(1)
Issue: 1
Pages: 8-20
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Amino and carboxyl terminal domains of Filamin-A interact with CRMP1 to mediate Sema3A-signaling2014
Author(s)
Fumio Nakamura, Kosuke Kumeta, Tomonobu Hida, Toshinari Isono, Yuichi Nakayama, Emiko Kuramata-Matsuoka, Naoya Yamashita, Yutaka Uchida, Ken-ichi Ogura, Keiko Gengyo-Ando, Shohei Mitani, Toshio Ogino, and Yoshio Goshima
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Journal Title
Nat. Commun.
Volume: 5 5325
Issue: 1
Pages: 1-14
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] rl8/ARL-8 functions in apoptotic cell removal by mediating phagolysosome formation in C. elegans.2013
Author(s)
Sasaki A, Nakae I, Nagasawa M, Hashimoto K, Abe F, Saito K, Fukuyama M, Gengyo-Ando K, Mitani S, Katada T, Kontani K.
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Journal Title
Mol. Biol. Cell
Volume: 24
Issue: 10
Pages: 1584-1592
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Neuronal mechanisms of decision making in C. elegans olfactory behavior revealed by a highly integrated microscope system2014
Author(s)
Tanimoto Y, Yamazoe A, Fujita K, Kawazoe Y, Miyanishi Y, Yamazaki SJ, Gengyo-Ando K, Nakai J, Fei X, Iwasaki Y, Hashimoto K, Kimura K
Organizer
第34回日本神経科学会大会
Place of Presentation
パシフィコ横浜(神奈川県・横浜市)
Year and Date
2014-11-09 – 2014-11-13
Related Report
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[Presentation] A neuronal mechanism for navigation along a repulsive odor gradient.2013
Author(s)
Akiko Yamazoe, Yuki Tanimoto, Kosuke Fujita, Yuya Kawazoe,Yosuke Miyanishi, Shuhei Yamazaki, Xianfeng Fei, Karl Emanuel Busch, Keiko Gengyo-Ando, Junichi Nakai, Yuichi Iino, Yuishi Iwasaki, Koichi Hashimoto, Kotaro Kimura
Organizer
The 19th international C. elegans meeting 2013
Place of Presentation
Los Angeles(USA)
Related Report
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[Presentation] A virtual reality running machine for worms-a highly integrated microscope system for olfactory behavior.2013
Author(s)
Yuki Tanimoto, Kosuke Fujita, Yuya Kawazoe, Yosuke Miyanishi, Shuhei Yamazaki, Xianfeng Fei, Karl Emanuel Busch, Keiko Gengyo-Ando, Junichi Nakai, Koichi Hashimoto, Kotaro Kimura.
Organizer
The 19th international C. elegans meeting 2013
Place of Presentation
Los Angeles(USA)
Related Report
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[Presentation] 線虫C. elegansの生殖系列細胞におけるGPIアンカー型タンパク質の解析2013
Author(s)
松田 采子, 村田 大輔, 野村 和子,秋好 紗弥香,出嶋 克史,Lance Wells,Karen L. Abbott,川崎 ナナ,安藤 恵子,中台 枝里子,三谷 昌平,野村 一也
Organizer
第36回日本分子生物学会年会
Place of Presentation
神戸ポートアイランド(神戸)
Related Report
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