Technology development for generation in vivo of organ using iPS cells after repair of the disease genome by TALEN
| Project/Area Number |
25640106
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical genome science
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| Research Institution | The University of Tokushima |
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Principal Investigator |
ISHIMARU Yoshiyasu 徳島大学, ソシオテクノサイエンス研究部, 学術研究員 (50435525)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | ゲノム編集 / CRISPR/Casシステム / TALEN / iPS細胞 / 胚盤胞補完法 / FGF10 / 臓器・器官再生 / ノックイン / CRISPR/Cas / 再生医療 |
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| Outline of Final Research Achievements |
Recently, gene knockout or knock-in technologies that disrupts genome sequence, the transcription activator-like effector nuclease (TALEN) system or the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system, have been developed and used to edit the genomes of various organisms. Our results demonstrate that CRISPR/Cas system more effectively elicits single-step biallelic mutations in mice. In addition, we hypothesized that targeted organs in deficient mice can be produced using a blastocyst complementation in a xenogenic environment. We therefore demonstrate that injection of mouse iPS cells into blastocysts derived from a mutant mouse strain in which the gene necessary to form a particular organ is deficient resulted in generation in vivo of organ almost entirely derived from injected mouse iPS cells.
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Report
(3 results)
Research Products
(2 results)
