Development and application of a micro-area illumination method for plants

• Project/Area Number: 25650098
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: Oyama Tokitaka 京都大学, 理学(系)研究科(研究院), 准教授 (30324396)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 光応答 / ルシフェラーゼ / 概日時計 / 生物発光 / ウキクサ / 概日リズム / 光照射 / cAMP

Outline of Final Research Achievements

We developed a technique that enables a micro-area illumination in a duckweed plant by fixing a tip of an optical fiber inside the plant body. This method should be used for the analysis about cell-to-cell light signal transmission: from light-perceived cells to cells without receiving light. To analyze the cell-to-cell light signal transmission in the circadian clock system, we developed a bioluminescence monitoring system at a single-cell level. This will be used in combination with the micro-area illumination method.

Research Products

• [Journal Article] Characterization of circadian rhythms of various duckweeds (2015)
  • Author(s): Muranaka, T., Okada, M., Yomo, J., Kubota, S., Oyama, T.
  • Journal Title: Plant Biology Volume: 17 Pages: 66-74
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Overexpression of a <i>CO</i> homologue disrupts the rhythmic expression of clock gene <i>LgLHYH1</i> in <i>Lemna gibba</i> (2014)
  • Author(s): Kumiko Ito-Miwa, Masayuki Serikawa, Takao Kondo, Tokitaka Oyama
  • Journal Title: Plant Biotechnology Volume: 31 Issue: 4 Pages: 319-328
  • DOI: 10.5511/plantbiotechnology.14.1111a
  • NAID: 130004704197
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of circadian rhythms of various duckweeds (2014)
  • Author(s): Muranaka, T., Okada, M., Yomo, J., Kubota, K., Oyama T.
  • Journal Title: Plant Biology Volume: in press
  • Peer Reviewed
• [Journal Article] A single-cell bioluminescence imaging system for monitoring cellular gene expression in a plant body. (2013)
  • Author(s): Muranaka T, Kubota S, Oyama T
  • Journal Title: Plant Cell Physiolsy Volume: 54(12) Issue: 12 Pages: 2085-2093
  • DOI: 10.1093/pcp/pct131
  • Peer Reviewed
• [Presentation] 単子葉植物のコウキクサにおけるCaMV35S ならびにZmUBQ プロモーターの転写活性のLUCIFERASE レポーターを用いた 時空間解析 (2016)
  • Author(s): 伊藤照悟，内海陽子，小山時隆
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] １細胞リズム測定から捉える植物の概日時計システム (2015)
  • Author(s): 村中智明，小山時隆
  • Organizer: 第22回日本時間生物学会学術大会
  • Place of Presentation: 東京大学
  • Year and Date: 2015-11-21
• [Presentation] ウキクサ植物の発光レポーター安定形質転換体を用いた概日リズム解析 (2015)
  • Author(s): 伊藤照悟，上野賢也，内海陽子，小山時隆
  • Organizer: 第22回日本時間生物学会学術大会
  • Place of Presentation: 東京大学
  • Year and Date: 2015-11-21
• [Presentation] 局所光照射装置を用いた植物概日時計システムにおける細胞間相互作用の解析 (2015)
  • Author(s): 四方純，村中智明，小山時隆
  • Organizer: 第22回日本時間生物学会学術大会
  • Place of Presentation: 東京大学
  • Year and Date: 2015-11-21
• [Presentation] Using duckweeds for basic research in plant physiology: Exploring plant biological clock systems (2015)
  • Author(s): Tokitaka Oyama
  • Organizer: The Third International Conference on Duckweed Research and Applications
  • Place of Presentation: 京都大学
  • Year and Date: 2015-07-03
  • Int'l Joint Research
• [Presentation] Developing a manipulation system of partial illumination to the microarea of duckweed plants for the detection of intercellular signaling on cellular circadian clocks (2015)
  • Author(s): Jun Yomo, Tomoaki Muranaka, masaaki Okada, Shogo Ito, Tokitaka Oyama
  • Organizer: The Third International Conference on Duckweed Research and Applications
  • Place of Presentation: 京都大学
  • Year and Date: 2015-07-03
  • Int'l Joint Research
• [Presentation] Improvement of fast and efficient Agrobacterium mediated stable transformation methods for Lemna species (2015)
  • Author(s): Shogo Ito, Yoko Utsumi and Tokitaka Oyama
  • Organizer: The Third International Conference on Duckweed Research and Applications
  • Place of Presentation: 京都大学
  • Year and Date: 2015-07-03
  • Int'l Joint Research
• [Presentation] Analysis of cellular circadian rhythms in the plant body for the detection of their physiological interaction (2015)
  • Author(s): 小山時隆
  • Organizer: Awaji-Symposium on RNA & CLOCK
  • Place of Presentation: 淡路夢舞台国際会議場（淡路市）
  • Year and Date: 2015-03-25 – 2015-03-26
• [Presentation] Transient and Agro-mediated stable transformation method for circadisn clock analysis in Lemna species (2015)
  • Author(s): 伊藤照悟, 小山時隆
  • Organizer: Awaji-Symposium on RNA & CLOCK
  • Place of Presentation: 淡路夢舞台国際会議場（淡路市）
  • Year and Date: 2015-03-25 – 2015-03-26
• [Presentation] Analysis of roles of ELF3 in the plant cellular circadian clock (2015)
  • Author(s): 岡田全朗, 小山時隆
  • Organizer: Awaji-Symposium on RNA & CLOCK
  • Place of Presentation: 淡路夢舞台国際会議場（淡路市）
  • Year and Date: 2015-03-25 – 2015-03-26
• [Presentation] ウキクサ類におけるカルス誘導とアグロバクテリウム共培養による安定形質転換体作製の試み (2015)
  • Author(s): 伊藤照悟, 内海陽子, 小山時隆
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] ウキクサ個体内における細胞概日時計の相互作用様式の解析 (2015)
  • Author(s): 村中智明, 小山時隆
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 植物細胞概日時計の明暗サイクル同調におけるELF3の機能 (2015)
  • Author(s): 岡田全朗, 小山時隆
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学（東京都）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 植物個体で単一細胞レベルの概日リズムを測定する技術とその応用 (2014)
  • Author(s): 小山時隆
  • Organizer: 第22回日本時間生物学会学術大会
  • Place of Presentation: 九州大学（福岡市）
  • Year and Date: 2014-11-08 – 2014-11-09
• [Presentation] ウキクサ植物が示す概日リズムの種間および種内多様性 (2014)
  • Author(s): 村中智明, 小山時隆
  • Organizer: 第22回日本時間生物学会学術大会
  • Place of Presentation: 九州大学（福岡市）
  • Year and Date: 2014-11-08 – 2014-11-09
• [Presentation] 光シグナルによる細胞概日時計リセットにおけるELF3の機能解析 (2014)
  • Author(s): 岡田全朗, 村中智明, 小山時隆
  • Organizer: 第22回日本時間生物学会学術大会
  • Place of Presentation: 九州大学（福岡市）
  • Year and Date: 2014-11-08 – 2014-11-09
• [Presentation] 局所的明暗条件に対する植物細胞概日時計の同期過程の時空間的解析 (2014)
  • Author(s): 四方純, 村中智明, 岡田全朗, 小山時隆
  • Organizer: 第22回日本時間生物学会学術大会
  • Place of Presentation: 九州大学（福岡市）
  • Year and Date: 2014-11-08 – 2014-11-09
• [Presentation] 一細胞発光測定で明らかにするウキクサにおける細胞概日振動子集団の振る舞い (2014)
  • Author(s): 村中智明、岡田全朗、小山時隆
  • Organizer: 第23回日本バイオイメージング学会学術集会
  • Place of Presentation: 大阪大学（吹田市）
  • Year and Date: 2014-09-05
• [Presentation] 細胞概日リズム可視化による植物細胞時計の時刻合わせ機構の解析 (2014)
  • Author(s): 岡田全朗、村中智明、小山時隆
  • Organizer: 第23回日本バイオイメージング学会学術集会
  • Place of Presentation: 大阪大学（吹田市）
  • Year and Date: 2014-09-05
• [Presentation] Characterization of cellular circadian rhythms in Lemna gibba by using an in vivo bioluminescence monitoring system (2013)
  • Author(s): Masaaki Okada, Tomoaki Muranaka, Toshihiro Yoshihara, Tokitaka Oyama
  • Organizer: The Second International Conference on Duckweed Research and Applications
  • Place of Presentation: Rutgers Univ., NJ, USA
• [Presentation] Use of duckweeds for physiological and genetic researches in the laboratory
  • Author(s): Tokitaka Oyama
  • Organizer: The Second International Conference on Duckweed Research and Applications
  • Place of Presentation: Rutgers Univ., NJ, USA
  • Invited
• [Presentation] Behavior of cellular circadian rhythms in plants
  • Author(s): Tokitaka Oyama
  • Organizer: Molecular clock 2014: Epigenetic Landscape in Biological Rhythms
  • Place of Presentation: Kyoto Univ. Kyoto
  • Invited
• [Presentation] Characterization of cellular circadian clocks in duckweeds
  • Author(s): Tomoaki Muranaka, Tokitaka Oyama
  • Organizer: The 2013 International Symposium on Plant Photobiology
  • Place of Presentation: Univ. Edinburgh, UK
• [Presentation] ウキクサ植物の細胞概日リズムにおけるELF3の機能解析
  • Author(s): 岡田全朗, 小山時隆
  • Organizer: 第20回日本時間生物学会学術大会
  • Place of Presentation: 近畿大学（東大阪キャンパス）
• [Presentation] ウキクサ植物における細胞概日振動子の挙動解析
  • Author(s): 中智明, 小山時隆
  • Organizer: 第20回日本時間生物学会学術大会
  • Place of Presentation: 近畿大学（東大阪キャンパス）
• [Presentation] 外環境の明暗周期に影響を受ける植物細胞概日リズムにおけるELF3の役割
  • Author(s): 岡田全朗, 村中智明, 小山時隆
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学
• [Presentation] ウキクサ植物にみられる概日リズム多様性
  • Author(s): 村中智明, 岡田全朗, 小山時隆
  • Organizer: 第55回日本植物生理学会年会
  • Place of Presentation: 富山大学