Development of novel metabolic engineering technology using one-carbon compounds and their metabolic regulatory systems

• Project/Area Number: 25660079
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Kyoto University
• Principal Investigator: YURIMOTO Hiroya 京都大学, (連合)農学研究科(研究院), 准教授 (00283648)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ホルムアルデヒド / メタノール / 遺伝子発現

Outline of Final Research Achievements

In order to develop novel metabolic engineering technology using one-carbon (C1) compounds and their metabolic regulatory systems, we investigated the metabolic flow of gene-engineered strains harboring the artificial bifunctional formaldehyde-fixation enzyme and molecular function of transcriptional regulators involved in regulation of C1 metabolism. Furthermore, we found that the sensor proteins localized in plasma membrane were involved in regulation of methanol-inducible gene expression in the methylotrophic yeast.

Research Products

• [Journal Article] Molecular characterization of Hap complex components responsible for methanol-inducible gene expression in the methylotrophic yeast Candida boidinii (2015)
  • Author(s): S. Oda, H. Yurimoto, N. Nitta, Y. Sasano, and Y. Sakai
  • Journal Title: Eukaryot. Cell Volume: 14 Issue: 3 Pages: 278-285
  • DOI: 10.1128/ec.00285-14
  • NAID: 120005593631
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Interactions of methylotrophs with plants and other hHeterotrophic bacteria (2015)
  • Author(s): H. Iguchi, H. Yurimoto, and Y. Sakai
  • Journal Title: Microorganisms Volume: 3 Issue: 2 Pages: 137-151
  • DOI: 10.3390/microorganisms3020137
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 植物から放出されるC1化合物と微生物―植物間相互作用による炭素循環 (2014)
  • Author(s): 由里本博也、阪井康能
  • Journal Title: AROMA RESEARCH Volume: 15 Pages: 80-84
• [Journal Article] C1微生物代謝経路の省エネ型炭素固定系としての利用とその問題点 (2013)
  • Author(s): 由里本博也、阪井康能
  • Journal Title: 生物工学会誌 Volume: 91 Pages: 384-387
• [Journal Article] C1微生物複合生物系が駆動する炭素循環（メタンサイクル） (2013)
  • Author(s): 由里本博也、井口博之、阪井康能
  • Journal Title: 生物の科学 遺伝 Volume: 67 Pages: 591-595
• [Journal Article] 葉面に棲息するC1微生物：共生系による炭素循環と環境バイオへの利用 (2013)
  • Author(s): 阪井康能、由里本博也
  • Journal Title: 環境バイオテクノロジー学会誌 Volume: 13 Pages: 79-84
• [Presentation] 植物から放出されるC1化合物と葉面微生物-植物間相互作用によるC1炭素循環 (2014)
  • Author(s): 由里本博也、阪井康能
  • Organizer: 第4回生物起源微量ガスワークショップ
  • Place of Presentation: 文部科学省研究交流センター（茨城県・つくば市）
  • Year and Date: 2014-11-20 – 2014-11-21
  • Invited
• [Presentation] メタノール誘導性遺伝子発現制御に関わるPpWsc1とPpWsc2の機能 (2014)
  • Author(s): 大澤晋、由里本博也、阪井康能
  • Organizer: 酵母遺伝学フォーラム第47回研究報告会
  • Place of Presentation: 東京大学（東京都・文京区）
  • Year and Date: 2014-09-01 – 2014-09-03
• [Presentation] Metabolic alteration by introducing bifunctional formaldehyde-fixing enzyme into the methylotrophic bacteria (2014)
  • Author(s): H. Yurimoto, D. Kajihara, and Y. Sakai
  • Organizer: Gordon Research Conference on Molecular basis of microbial one-carbon metabolism
  • Place of Presentation: South Hadley, MA（米国）
  • Year and Date: 2014-08-10 – 2014-08-15
• [Presentation] Functional analysis of the Wsc family proteins related to methanol-inducible gene expression in methylotrophic yeasts (2014)
  • Author(s): S. Osawa, H. Yurimoto, and Y. Sakai
  • Organizer: Gordon Research Conference on Molecular basis of microbial one-carbon metabolism
  • Place of Presentation: South Hadley, MA（米国）
  • Year and Date: 2014-08-10 – 2014-08-15
• [Presentation] 微生物ホルムアルデヒド代謝の戦略的活用 (2014)
  • Author(s): 由里本博也、阪井康能
  • Organizer: 日本農芸化学会2014年度大会シンポジウム 「アルデヒドのバイオサイエンス ―生物の多様なアルデヒド代謝系とその戦略的活用―」
  • Place of Presentation: 明治大学（川崎市）
  • Invited
• [Presentation] Metabolic alteration by introducing bifunctional formaldehyde-fixing enzyme into the methylotrophic bacterium (2013)
  • Author(s): H. Yurimoto, D. Kajihara, and Y. Sakai
  • Organizer: V International Conference on Environmental, Industrial and Applied Microbiology
  • Place of Presentation: マドリッド（スペイン）
  • Invited
• [Presentation] 植物から放出されるC1化合物と微生物－植物間相互作用による炭素循環
  • Author(s): 由里本博也、阪井康能
  • Organizer: 第252回生存圏シンポジウム 「植物アロマのメタ代謝科学 ～生態学、大気科学、植物科学の融合～」
  • Place of Presentation: 京都大学（宇治市）
  • Invited