The clarification and application of a novel regulatory mechanism of cholesterol metabolism mediated by polyphenols.
| Project/Area Number |
25660100
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Food science
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| Research Institution | Gifu University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | ポリフェノール / カテキン / コレステロール / 低密度リポタンパク質受容体 / アネキシンA2 / PCSK9 / EGCG |
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| Outline of Final Research Achievements |
Interestingly, EGCG treatment for 3, 6, 12 or 18 hours induced a drastic decrease in extracellular PCSK9 level without reduction of PCSK9 mRNA level in HepG2 cells. EGCG treatment for 24 hours induced a significant decrease in extracellular PCSK9 level accompanying with an up-regulation of LDL receptor. EGCG increased LDL receptor mRNA level through the extracellular signal-related kinase and p38 mitogen-activated protein kinase signalling pathways. We found for the first time that EGCG induced a suppression of PCSK9 accompanying with an up-regulation of LDL receptor in HepG2 cells. Furthermore, EGCG induced an activation of LDL receptor or a decrease of PCSK9 level is partially mediated by Annexin A2.
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Report
(4 results)
Research Products
(4 results)
