| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Research Abstract |
In enzymatic saccharification of cellulosic biomass, it is necessary to decrease the dosage of cellulase. However, it is known that lignin is bound to the cellulase during the saccharification, and inactivates the enzyme. In this research, we modified surface environment of the enzyme to establish the methodology to develop the high performance cellulase having suppressed absorptivity to lignin. We expressed three endoglucanse, EG1, EG2 and EG3 from Trichoderma reesei in Pichia pastris, and analyzed effects of depletion of CBM and carbohydrate chains of the enzymes on the absorptivity to the lignin.
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