Chemical hazard assessment with the genome-nuclear receptor interaction array system
Project/Area Number |
25660228
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Veterinary medical science
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Research Institution | Ehime University |
Principal Investigator |
IWATA Hisato 愛媛大学, 沿岸環境科学研究センター, 教授 (10271652)
|
Research Collaborator |
YOSHINOUCHI Yuka 愛媛大学, 大学院理工学研究科博士前期課程, 学生
HIRANO Masashi 愛媛大学, 沿岸環境科学研究センター, 特任助教
KIM Eun-Young Kyung Hee University, Department of Biology, Associate Professor
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
Keywords | 核内受容体 / ビスフェノール類 / 水酸化ポリ塩化ビフェニル / 化学物質 / スクリーニング / 種差 |
Outline of Final Research Achievements |
The aim of this study is to construct an in vitro assay system to screen hazardous environmental chemicals that can transactivate the gene via Baikal seal estrogen receptors (bsERs). We constructed the in vitro reporter gene assay system where a expression vector containing bsERα or bsERβ cDNA and a reporter vector containing ER response element were introduced in COS-1 cells. Using this in vitro assay system, we screened the transactivation potencies of 26 bisphenol A and its analogues (BPs) and 16 hydroxylated polychlorinated biphenyl congeners (OH-PCBs). The results showed that most of the test chemicals were able to activate bsERα and bsERβ.
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Report
(4 results)
Research Products
(91 results)
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[Journal Article] Organohalogen compounds in pet dog and cat: Do pets biotransform natural brominated products in food to harmful hydroxlated substances?2016
Author(s)
Mizukawa H, Nomiyama K, Nakatsu S, Iwata H, Yoo J, Kubota A, Yamamoto M, Ishizuka M, Ikenaka Y, Nakayama M.M.S, Kunisue T, Tanabe S
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Journal Title
Environmental Science & Technology
Volume: 50
Issue: 1
Pages: 444-452
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Auto-induction mechanism of aryl hydrocarbon receptor 2 (AHR2) gene by TCDD-activated AHR1 AHR2 in the red seabream (Pagrus major)2016
Author(s)
Bak, S. M., Iida, M., Soshilov, A. A., Denison, M. S., Iwata, H. and Kim, E. Y.
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Journal Title
Archives of Toxicology
Volume: 印刷中
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Haptoglobin genotyping of Vietnamese: global distribution of HPdel, complete deletion allele of the HP gene.2015
Author(s)
Soejima M, Agusa T, Iwata H, Fujihara J, Kunito T, Takeshita H, Lan VT, Minh TB, Takahashi S, Trang PT, Viet PH, Tanabe S, Koda Y.
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Journal Title
Legal Medicine
Volume: 17
Issue: 1
Pages: 14-16
DOI
Related Report
Peer Reviewed
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[Journal Article] Relationship between Arsenic (+3 Oxidation State) Methyltransferase Genetic Polymorphisms and Methylation Capacity of Inorganic Arsenic2015
Author(s)
阿草哲郎, 國頭 恭, Nguyen Minh Tue, Vi Thi Mai Lan, Tu Binh Minh, Pham Thi Kim Trang, 藤原純子, 竹下治男, 高橋 真, Pham Hung Viet, 田辺信介, 岩田久人
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Journal Title
Nippon Eiseigaku Zasshi (Japanese Journal of Hygiene)
Volume: 70
Issue: 3
Pages: 186-196
DOI
NAID
ISSN
0021-5082, 1882-6482
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Effects of persistent organochlorine exposure on the liver transcriptome of the common minke whale (Balaenoptera acutorostrata) from the North Pacific.2014
Author(s)
Niimi, S., Imoto, M., Kunisue, T., Watanabe, M.X., Kim, E.Y., Nakayama, K., Yasunaga, G., Fujise, Y., Tanabe, S., Iwata, H.
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Journal Title
Ecotoxicology and Environmental Safety
Volume: 108
Pages: 95-105
DOI
Related Report
Peer Reviewed
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[Journal Article] Transient suppression of AHR activity in early red seabream embryos does not prevent the disruption of peripheral nerve projection by 2,3,7,8-tetrachlorodibenzo-p-dioxin.2014
Author(s)
Iida, M., Bak, S.M., Murkami, Y., Kim, E.Y., Iwata, H.
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Journal Title
Aquatic Toxicology
Volume: 154
Pages: 39-47
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Molecular and functional characterization of a novel aryl hydrocarbon receptor isoform, AHR1β in the chicken (Gallus gallus)2013
Author(s)
Lee, J. S., Iwabuchi, K., Nomaru, K., Nagahama, N., Kim, E. Y., Iwata, H
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Journal Title
Toxicological Sciences
Volume: 136(2)
Issue: 2
Pages: 450-466
DOI
Related Report
Peer Reviewed
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[Journal Article] Quantitative analysis of the interaction of constitutive androstane receptor with chemicals and steroid receptor coactivator 1 using surface Plasmon resonance biosensor systems : A case study of the Baikal seal (Pusa sibirica) and mouse.2013
Author(s)
Dau, P.T., Sakai, H., Hirano. M., Ishibashi, H., Tanaka, Y., Kameda, K., Fujino, T., Kim, E,Y. and Iwata, H.
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Journal Title
Toxicol. Sci.
Volume: 131(1)
Issue: 1
Pages: 116-127
DOI
Related Report
Peer Reviewed
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[Presentation] Assessment of effects of dioxins in the Baikal seal (Pusa Sibirica) by proteome and transcriptome analyses2016
Author(s)
Lauan, M. C., Yoo, J., Agusa, T., Hirano, M., Kim, E. Y. and Iwata, H.
Organizer
International Symposium of Environmental Chemistry and toxicology - To Accelerate a Global Network of Environmental Researchers
Place of Presentation
愛媛大学(愛媛県松山市)
Year and Date
2016-03-19
Related Report
Int'l Joint Research
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[Presentation] Effects of a naturally occurring dioxin, 1,3,7-tribromodibenzo-p-dioxin on the mysid transcriptome2016
Author(s)
Hirano, M., Uchida, M., Kusano, T., Arizono, K. and Iwata, H.
Organizer
International Symposium of Environmental Chemistry and toxicology - To Accelerate a Global Network of Environmental Researchers
Place of Presentation
愛媛大学(愛媛県松山市)
Year and Date
2016-03-19
Related Report
Int'l Joint Research
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[Presentation] Liver transcriptome analysis to assess the effects of prenatal exposure to bisphenol a of rat offspring2016
Author(s)
Hoa, T. N., Yamamoto, K., Agusa, T., Hirano, M., Ochiai, M., Kim, E. Y. and Iwata, H.
Organizer
International Symposium of Environmental Chemistry and toxicology - To Accelerate a Global Network of Environmental Researchers
Place of Presentation
愛媛大学(愛媛県松山市)
Year and Date
2016-03-19
Related Report
Int'l Joint Research
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[Presentation] Ecological factors drive natural selection pressure affecting avian dioxin receptor genotypes2016
Author(s)
Hwang, J. H., Park, J. Y., Park, H. J., Bak, S. M., Hirano, M., Iwata, H., Park, Y. S. and Kim, E. Y.
Organizer
International Symposium of Environmental Chemistry and toxicology - To Accelerate a Global Network of Environmental Researchers
Place of Presentation
愛媛大学(愛媛県松山市)
Year and Date
2016-03-19
Related Report
Int'l Joint Research
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[Presentation] Transcriptome analysis to identify adverse outcome pathways mediated by the aryl hydrocarbon receptor in TCDD-treated red seabream embryos2016
Author(s)
Iida, M., Fujii, S., Uhida, M., Nakamura, H., Kagami, Y., Agusa, T., Hirano, M., Bak, S. M., Kim, E. Y. and Iwata, H.
Organizer
International Symposium of Environmental Chemistry and toxicology - To Accelerate a Global Network of Environmental Researchers
Place of Presentation
愛媛大学(愛媛県松山市)
Year and Date
2016-03-19
Related Report
Int'l Joint Research
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[Presentation] Effect of prenatal exposure of BPA on the transcriptome in chicken embryos2016
Author(s)
Itoh, S., Agusa, T., Hirano, M., Ochiai, M., Kim, E. Y. and Iwata, H.
Organizer
International Symposium of Environmental Chemistry and toxicology - To Accelerate a Global Network of Environmental Researchers
Place of Presentation
愛媛大学(愛媛県松山市)
Year and Date
2016-03-19
Related Report
Int'l Joint Research
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[Presentation] Hepatic transcriptome analysis to assess the effects of polychlorinated biphenyls on beagle dogs2016
Author(s)
Tamura, S., Yoo, J., Mizukawa, H., Agusa, T., Hirano, M., Eguchi A., Nomiyama, K., Tanabe, S., Kim, E. Y. and Iwata, H.
Organizer
International Symposium of Environmental Chemistry and toxicology - To Accelerate a Global Network of Environmental Researchers
Place of Presentation
愛媛大学(愛媛県松山市)
Year and Date
2016-03-19
Related Report
Int'l Joint Research
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[Presentation] Assessment of metabolic potential of polyhlorinated biphenyl congeners in the beagle dog liver by in vitro and in silico approaches2016
Author(s)
Yoo, J., Hirano, M., Nomiyama, K., Kim, E. Y., Tanabe, S. and Iwata, H.
Organizer
International Symposium of Environmental Chemistry and toxicology - To Accelerate a Global Network of Environmental Researchers
Place of Presentation
愛媛大学(愛媛県松山市)
Year and Date
2016-03-19
Related Report
Int'l Joint Research
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[Presentation] Structural preference of OH-PCBs to activate the Baikal Seal estrogen receptors2016
Author(s)
Yoshinouchi, Y., Hirano, M., Nomiyama, K., Tanabe, S., Kim, E. Y. and Iwata, H.
Organizer
International Symposium of Environmental Chemistry and toxicology - To Accelerate a Global Network of Environmental Researchers
Place of Presentation
愛媛大学(愛媛県松山市)
Year and Date
2016-03-19
Related Report
Int'l Joint Research
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[Presentation] Effects of prenatal exposure to bisphenol A on the liver transcriptome of rat offspring2015
Author(s)
Hoa, N. T., Yamamoto, K., Agusa, T., Hirano, M., Ochiai, M., Kim, E. Y. and Iwata, H.
Organizer
環境ホルモン学会第18回研究発表会
Place of Presentation
自治医科大学地域医療学情報センター(栃木県下野市)
Year and Date
2015-12-10
Related Report
-
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[Presentation] Hepatic cytochrome P450-dependent metabolic potency of polychlorinated biphenyls in the baikal seal (Pula sibirica)2015
Author(s)
Yoo, J., Hirano, M., Mizukawa, H., Nomiyama, K., Agusa, T., Kim, E. Y., Tanabe, S. and Iwata, H.
Organizer
18th International Symposium on Pollutant Responses in Marine Organisms
Place of Presentation
クラリオンホテル&コングレス(トロンハイム、ノルウェイ)
Year and Date
2015-05-25
Related Report
Int'l Joint Research
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[Presentation] Identification of functional xenobiotic-responsive elements controlling AHR2 expression of red seabream (Pagrus major)2015
Author(s)
Bak, S. M., Iida, M., Soshilov, A. A., Denison, M. S., Iwata, H. and Kim, E. Y.
Organizer
18th International Symposium on Pollutant Responses in Marine Organisms
Place of Presentation
クラリオンホテル&コングレス(トロンハイム、ノルウェイ)
Year and Date
2015-05-25
Related Report
Int'l Joint Research
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[Presentation] Hepatic proteome analysis of baikal seals contaminated with dioxins and related compounds2015
Author(s)
Lauan, M. C., Agusa, T., Hirano, M., Iwata, H., Kim, E. Y. and Yoo, J.
Organizer
18th International Symposium on Pollutant Responses in Marine Organisms
Place of Presentation
クラリオンホテル&コングレス(トロンハイム、ノルウェイ)
Year and Date
2015-05-25
Related Report
Int'l Joint Research
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[Presentation] Comparison of hepatic cytochrome P450-dependent metabolic potencies of polychlorinated biphenyls in the caniformia, the baikal seal and the beagle dog.2015
Author(s)
Yoo, J., Mizukawa, H., Nomiyama, K., Kim, E. Y., Tanabe, S. and Iwata, H.
Organizer
18th International Symposium on Pollutant Responses in Marine Organisms
Place of Presentation
クラリオンホテル&コングレス(トロンハイム、ノルウェイ)
Year and Date
2015-05-25
Related Report
Int'l Joint Research
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[Presentation] In vitro and in silico assessment of transactivation potencies of baikal seal estrogen receptors2015
Author(s)
Yoshinouchi, Y., Shimizu, S., Lee, J. S., Hirano, M., Agusa, T., Suzuki, K. T., Nakata, H., Kim, E. Y. and Iwata, H.
Organizer
18th International Symposium on Pollutant Responses in Marine Organisms
Place of Presentation
クラリオンホテル&コングレス(トロンハイム、ノルウェイ)
Year and Date
2015-05-25
Related Report
Int'l Joint Research
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[Presentation] Ecological factors affecting natural selection of avian AHR1 genotypes2015
Author(s)
Hwang, J. H., Park, H. J., Bak, S. M., Hirano, M., Iwata, H., Park, Y. S. and Kim, E. Y.
Organizer
18th International Symposium on Pollutant Responses in Marine Organisms
Place of Presentation
クラリオンホテル&コングレス(トロンハイム、ノルウェイ)
Year and Date
2015-05-25
Related Report
Int'l Joint Research
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[Presentation] Relationship between mRNA expressionlevel and SNPs of As3MT in residents from arsenic-contaminated areas in Vietnam.2015
Author(s)
Agusa, T., Kunito, T., Tue, N.M., Lan, V.T., Minh, T.B., Trang, P.T., Fujihara, J., Takeshita, H., Takahashi, S., Viet, P.H., Tanabe, S., Iwata, H.
Organizer
Society of Toxicology (SOT) 54th Annual Meeting
Place of Presentation
サンディエゴ、アメリカ
Year and Date
2015-03-25
Related Report
-
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[Presentation] Strain-differences in the proteome of dioxin-sensitive and -resistant mice treated with 2,3,7,8-tetrabromodibenzo-p-dioxin.2015
Author(s)
Nguyen, T., Lauan, M., Yoo, J., Iida, M., Agusa, T., Kim, E.Y., Miyazaki, T., Nose, M., Iwata, H.
Organizer
Society of Toxicology (SOT) 54th Annual Meeting
Place of Presentation
サンディエゴ、アメリカ
Year and Date
2015-03-24
Related Report
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[Presentation] RNA-Seq analysis of the mysid crustacean transcriptome treated with naturally occurring 1,3,7-tribromodibenzo-p-dioxin.2015
Author(s)
Hirano, M., Uchida, M., Kusano, T., Arizono, K., Iwata, H.
Organizer
Society of Toxicology (SOT) 54th Annual Meeting
Place of Presentation
サンディエゴ、アメリカ
Year and Date
2015-03-23
Related Report
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[Presentation] Effects of a naturally occurring dioxin, 1,3,7-tribromodibenzo-p-dioxin on the mysid transcriptome2014
Author(s)
Hirano, M., Uchida, M., Kusano, T., Arizono, K., Iwata, H.
Organizer
環境ホルモン学会 第17回研究発表会
Place of Presentation
東京都文京区、日本
Year and Date
2014-12-10
Related Report
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[Presentation] Ecological factors as pressure of natural selection of AhR genotypes in avian species2014
Author(s)
Hwang, J.H., Park, H.J., Park, J.Y., Bak, S.M., Park, Y.S., Hirano, M., Kim, E.Y., Iwata, H.
Organizer
DIOXIN Madrid 2014 / 34th International Symposium on Halogenated Persistent Organic Pollutants
Place of Presentation
マドリード、スペイン
Year and Date
2014-09-04
Related Report
-
[Presentation] In vitro and in silico assessment of transactivation potencies of Baikal seal estrogen receptors αand β by persistent organic pollutants and bisphenols2014
Author(s)
Yoshinouchi, Y., Shimizu, S., Suzuki, K., Lee, J.S., Nakata, H., Hirano, M., Kim, E.Y., Agusa, T., Iwata, H.
Organizer
DIOXIN Madrid 2014 / 34th International Symposium on Halogenated Persistent Organic Pollutants
Place of Presentation
マドリード、スペイン
Year and Date
2014-09-04
Related Report
-
[Presentation] Effects of polychlorinated biphenyls on the expression of cytochrome P450s in beagle dogs2014
Author(s)
Tamura, S., Yoo, J., Nomiyama, K., Mizukawa, H., Tanabe, S., Agusa, T., Kim, E.Y., Hirano, M., Iwata, H.
Organizer
DIOXIN Madrid 2014 / 34th International Symposium on Halogenated Persistent Organic Pollutants
Place of Presentation
マドリード、スペイン
Year and Date
2014-09-04
Related Report
-
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[Presentation] AS3MT遺伝子多型と無機ヒ素のメチル化能力との関係2014
Author(s)
阿草哲郎, 國頭 恭, Nguyen Minh Tue, Vi Thi Mai Lan, Tu Binh Minh, Pham Thi Kim Trang, 藤原純子, 竹下治男, 高橋 真, Pham Hung Viet, 田辺信介, 岩田久人
Organizer
第84回日本衛生学会学術総会
Place of Presentation
岡山県岡山市、日本
Year and Date
2014-05-25
Related Report
Invited
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[Presentation] Identification of quantitative trait loci (QTL) associated with inorganic arsenic metabolism using recombinant inbred strains of mice2013
Author(s)
Agusa, T., Komori, H., Soga, Y., Nose, M., Mori, S., Kubota, R., Tanabe, S. and Iwata, H.
Organizer
The XIII International Congress of Toxicology 2013
Place of Presentation
Seoul, Korea
Related Report
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[Presentation] Identification and functional characterization of a novel chicken aryl hydrocarbon receptor isoform, 2nd AHR12013
Author(s)
Lee, J. S., Iwabuchi, K., Nomaru, K., Nagahama, N., Kim, E. Y. and Iwata, H.
Organizer
The XIII International Congress of Toxicology 2013
Place of Presentation
Seoul, Korea
Related Report
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[Presentation] Effects of aryl hydrocarbon receptor and transcriptional co-factor(s) on transactivation in MRL/lpr and C3H/lpr mouse strains2013
Author(s)
Cho, S. W., Kim, E. Y., Suzuki, K., Miura, Y., Iwata, H., Miyazaki, T. and Nose, M.
Organizer
The XIII International Congress of Toxicology 2013
Place of Presentation
Seoul, Korea
Related Report
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[Presentation] Effect of aryl hydrocarbon receptor variants and hnRNPA2/B1 on dioxin-induced cyp1a1 transactivation in MRL/lpr and C3H/lpr2013
Author(s)
Cho, S. W., Suzuki, K. I., Miura, Y., Watanabe, M., Miyazaki, T., Nose, M., Iwata, H. and Kim, E. Y.
Organizer
The 33rd International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN2013)
Place of Presentation
Daegu, Korea
Related Report
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[Presentation] Thyroid hormone-mediated effects of dioxins in wild Baikal seals (Pusa sibirica)2013
Author(s)
Iwata, H., Hirakawa, S., Eguchi, A., Imaeda, D., Kunisue, T., Kim, E. Y., Kannan, K., Petrov, E. A., Batoev, V. B. and Tanabe, S.
Organizer
The 33rd International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN2013)
Place of Presentation
Daegu, Korea
Related Report
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[Presentation] Polychlorinated biphenyls and their hydroxylated metabolites (OH-PCBs) in the Baikal seals and implication from toxicogenomic approach2013
Author(s)
Nomiyama, K., Eguchi, A., Hirakawa, S., Kanbara, C., Imaeda, D., Kim, E. Y., Iwata, H. and Tanabe, S.
Organizer
The 33rd International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN2013)
Place of Presentation
Daegu, Korea
Related Report
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[Presentation] Functional characterization of aryl hydrocarbon receptor from the polar bear (Ursus maritimus)2013
Author(s)
Hwang, J. H., Jeong, L. R., Kannan, K., Evans T. J., Iwata, H. and Kim, E. Y.
Organizer
The 33rd International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN2013)
Place of Presentation
Daegu, Korea
Related Report
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