Assembly of baculovirus genome DNA fragments in yeast for improving baculovectors
| Project/Area Number |
25660260
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Insect science
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| Research Institution | Hokkaido University |
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Principal Investigator |
BANDO Hisanori 北海道大学, (連合)農学研究科(研究院), 教授 (20189731)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Masanao 基礎生物学研究所, 発生生物学領域, 助教 (20517693)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 昆虫機能利用 / 有用物質生産 |
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| Outline of Final Research Achievements |
The technologies that enable flexible manipulation of virus genome are very important technical base for improving viral vectors. The aim of this study is to establish a method for assembling baculovirus genome DNA from both synthetic and natural DNA fragments to improve baculovectors. First, we constructed a baculovirus of the silkworm (BmNPV/bacmid) carrying an yeast replication origin (Yori). Second, we cloned 21 overlapping DNA fragments covering whole sequence of the BmNPV/bacmid genome DNA with Yori in bacterial cells using a plasmid vector. Lastly, we assembled the 21 fragments in yeast to form the complete BmNPV genome DNA.
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Report
(3 results)
Research Products
(1 results)
