An attempt to produce a transgenic medaka that express a cilia-related gene in a spatiotemporally regulated manner

Research Project

Project/Area Number	25670098
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	General anatomy (including histology/embryology)
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	KOBAYASHI DAISUKE 京都府立医科大学, 医学研究科, 助教 (60376548)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	発生 / 繊毛 / 嚢胞腎 / 遺伝子発現制御
Outline of Final Research Achievements	I have attempted to produce a transgenic medaka that express a cilia-related gene in a spatiotemporally regulated manner by combination of Transcription Activator-Like Effector Nuclease (TALEN) method, Cre/loxP system and heat-shock promoter. I have already achieved that a TALEN knockout medaka in which a cilia-related gene in knocked out. This fish line developed a polycystic kidney as expected. Cystic dilation was already detected at hatching and gradually increased in size as fish grew. This phenotype resembles that of human polycystic kidney disease. Construction of a transgenic line using kidney specific promoter in progress.

Report

(3 results)